Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru-SP, Zip code: 17012-901, Brazil.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru-SP, Zip code: 17012-901, Brazil.
J Dent. 2021 Aug;111:103732. doi: 10.1016/j.jdent.2021.103732. Epub 2021 Jun 24.
To evaluate the effect of experimental solutions containing TiF/NaF and chitosan on bacterial species and on enamel caries prevention.
Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for five days, under 5% CO and 37 °C. From the second day until the end, the treatments were applied (1 × 60 s/day): (1) NaF (500 ppm F, positive control); (2) TiF and NaF (TiF: 190 ppm Ti and 300 ppm F; NaF: 190 ppm F); (3) similar to 2 plus 0.5% chitosan (Ch 500 mPas, 75% deacetylation); (4) phosphate buffer solution (negative control); and (5) 0.5% chitosan (Ch 500 mPas, 75% deacetylation). CFU counting was performed for total microorganism, total streptococcus, total lactobacillus and Streptococcus mutans. Enamel demineralization was measured by transverse microradiography-TMR. The data were compared using ANOVA/Tukey or Kruskal-Wallis/Dunn tests (p < .050).
No differences were found between the treatments with respect to CFU counting (ANOVA, p > .050). Enamel treated with TiF/NaF plus chitosan solution presented the lowest demineralization compared to the negative control and pure chitosan solution. On the other hand, this experimental solution did not significantly differ from TiF/NaF and NaF solutions, being all of them able to significantly reduce mineral loss (50-74%), but only TiF/NaF plus chitosan reduced lesion depth (55%) compared to the negative control (p = .001).
TiF/NaF plus chitosan solution had no antimicrobial effect, but it was able to reduce enamel caries development in 79% compared to control under this model.
This study showed that TiF/NaF plus chitosan solution had no antimicrobial effect, but it was able to reduce enamel caries development under a microcosm biofilm model.
评估含 TiF/NaF 和壳聚糖的实验溶液对细菌种类和预防釉质龋的影响。
在 5% CO 和 37°C 下，将人唾液与 McBain 唾液（0.2%蔗糖）混合在牛牙釉质上产生微宇宙生物膜，持续五天。从第二天到结束，每天应用以下处理方法（1×60s/天）：（1）NaF（500ppmF，阳性对照）；（2）TiF 和 NaF（TiF：190ppmTi 和 300ppmF；NaF：190ppmF）；（3）类似于 2 加上 0.5%壳聚糖（Ch500mPas，75%脱乙酰度）；（4）磷酸盐缓冲溶液（阴性对照）；和（5）0.5%壳聚糖（Ch500mPas，75%脱乙酰度）。对总微生物、总链球菌、总乳杆菌和变形链球菌进行 CFU 计数。通过横向显微射线照相法（TMR）测量牙釉质脱矿。使用方差分析/Tukey 或 Kruskal-Wallis/Dunn 检验（p<.050）比较数据。
在 CFU 计数方面，各处理之间没有差异（方差分析，p>.050）。与阴性对照和纯壳聚糖溶液相比，用 TiF/NaF 加壳聚糖溶液处理的牙釉质脱矿程度最低。另一方面，该实验溶液与 TiF/NaF 和 NaF 溶液没有显著差异，所有这些溶液都能够显著减少矿物质损失（50-74%），但只有 TiF/NaF 加壳聚糖溶液能够降低病变深度（55%）与阴性对照相比（p=.001）。
TiF/NaF 加壳聚糖溶液没有抗菌作用，但在该模型下，与对照相比，它能够将釉质龋的发展减少 79%。
本研究表明，TiF/NaF 加壳聚糖溶液没有抗菌作用，但在微宇宙生物膜模型下，它能够减少釉质龋的发展。
