Souza Beatriz Martines de, Fernandes Neto Constantino, Salomão Priscila Maria Aranda, Vasconcelos Layla Reginna Silva Munhoz de, Andrade Flaviana Bombarda de, Magalhães Ana Carolina
Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo, Brasil.
Departamento de Dentística, Endodontia e Materiais Odontológicos, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo, Brasil.
J Appl Oral Sci. 2018;26:e20170304. doi: 10.1590/1678-7757-2017-0304. Epub 2018 Feb 22.
Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect.
This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model.
A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography - TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p<0.05).
Only chlorhexidine significantly increased the number of dead bacteria (68.8±13.1% dead bacteria) compared to untreated control (48.9±16.1% dead bacteria). No treatment reduced the CFU counting (total microorganism and total streptococci) compared to the negative controls. Only TiF4 was able to reduce enamel demineralization (ΔZ 1110.7±803.2 vol% μm) compared to both negative controls (untreated: ΔZ 4455.3±1176.4 vol% μm).
TiF4 varnish has no relevant antimicrobial effect. Nevertheless, TiF4 varnish was effective in reducing enamel demineralization under this model.
四氟化钛（TiF4）因能与牙釉质相互作用减少脱矿而闻名。然而，关于其潜在抗菌作用的信息却尚无报道。
本研究使用牙科微生态生物膜模型，评估了TiF4 清漆与氟化钠清漆、氯己定凝胶（阳性对照）、安慰剂清漆和未处理组（阴性对照）相比的抗菌和防龋潜力。
在先前用清漆处理过的牛牙釉质上形成微生态生物膜，接种来自人类唾液与麦克贝恩唾液混合的菌液，在0.2%蔗糖环境下培养14天。所有实验均进行三次生物学重复（每次实验每组n = 4）。评估的因素包括：细菌活力（死菌和活菌百分比）；菌落形成单位计数（log10 CFU/mL）；以及牙釉质脱矿（横向显微放射照相术 - TMR）。数据采用方差分析/图基检验或克鲁斯卡尔 - 沃利斯/邓恩检验进行分析（p < 0.05）。
与未处理对照（48.9±16.1%死菌）相比，只有氯己定能显著增加死菌数量（68.8±13.1%死菌）。与阴性对照相比，没有处理能减少菌落形成单位计数（总微生物和总链球菌）。与两个阴性对照（未处理：ΔZ 4455.3±1176.4 vol% μm）相比，只有TiF4 能够减少牙釉质脱矿（ΔZ 1110.7±803.2 vol% μm）。
TiF4 清漆没有相关的抗菌作用。然而，在该模型下TiF4 清漆在减少牙釉质脱矿方面是有效的。
