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牙龈卟啉单胞菌脂多糖、炎症细胞因子和前列腺素 E 改变人牙龈成纤维细胞中 SARS-CoV-2 进入和加工基因的表达。

Altered expression of SARS-CoV-2 entry and processing genes by Porphyromonas gingivalis-derived lipopolysaccharide, inflammatory cytokines and prostaglandin E in human gingival fibroblasts.

机构信息

Department of Periodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.

出版信息

Arch Oral Biol. 2021 Sep;129:105201. doi: 10.1016/j.archoralbio.2021.105201. Epub 2021 Jun 21.

DOI:10.1016/j.archoralbio.2021.105201
PMID:34174588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8215882/
Abstract

OBJECTIVE: The aim of this in vitro study was to investigate the expression of SARS-CoV-2 entry and processing genes in human gingival fibroblasts (HGnF) following treatment with Porphyromonas gingivalis-derived lipopolysaccharide (PgLPS) or inflammatory cytokines/mediators. DESIGN: We assessed the expression of SARS-CoV-2 entry and processing genes; angiotensin-converting enzyme 2 (ACE2), cellular serine proteases transmembrane serine protease 2 (TMPRSS2), Furin, and basigin (BSG) in HGnF by real-time PCR. To further asses the contribution of PgLPS and inflammatory cytokines/mediators to proliferation and SARS-CoV-2 entry and processing gene expression, HGnF were treated with PgLPS, IL1β, TNFα, and PGE. RESULTS: The expression for ACE2 in HGnF was significantly elevated after PgLPS or IL1β, TNFα, PGE treatment. The expression of TMPRSS2 was increased by PgLPS, IL1β, or PGE while BSG was elevated by PgLPS and IL1β. The expression of BSG and FURIN decreased after TNFα treatment. CONCLUSION: SARS-CoV-2 entry and processing genes are expressed in human gingival fibroblasts and their expressions are altered by PgLPS, IL1β, TNFα and PGE treatment.

摘要

目的：本体外研究旨在探讨牙龈成纤维细胞（HGnF）在牙龈卟啉单胞菌衍生脂多糖（PgLPS）或炎症细胞因子/介质处理后，SARS-CoV-2 进入和加工基因的表达情况。

设计：我们通过实时 PCR 评估了 SARS-CoV-2 进入和加工基因，包括血管紧张素转换酶 2（ACE2）、细胞丝氨酸蛋白酶跨膜丝氨酸蛋白酶 2（TMPRSS2）、弗林蛋白酶和基底膜糖蛋白（BSG）在 HGnF 中的表达。为了进一步评估 PgLPS 和炎症细胞因子/介质对 HGnF 增殖和 SARS-CoV-2 进入和加工基因表达的影响，我们用 PgLPS、IL1β、TNFα 和 PGE 处理 HGnF。

结果：PgLPS 或 IL1β、TNFα、PGE 处理后，HGnF 中 ACE2 的表达明显升高。TMPRSS2 的表达在 PgLPS、IL1β 或 PGE 作用下增加，而 BSG 在 PgLPS 和 IL1β 作用下升高。BSG 和 FURIN 的表达在 TNFα 处理后降低。

结论：SARS-CoV-2 进入和加工基因在人牙龈成纤维细胞中表达，其表达受 PgLPS、IL1β、TNFα 和 PGE 处理的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/3033fe955385/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/104bfa51e5cd/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/092083f85217/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/da83bb156475/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/3a6e6e5cc387/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/3033fe955385/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/104bfa51e5cd/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/092083f85217/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/da83bb156475/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/3a6e6e5cc387/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352c/8215882/3033fe955385/gr5_lrg.jpg

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[2]
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[3]
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Front Dent Med. 2024-9-4

[4]
Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients.

Sci Rep. 2025-2-6

[5]
Inhibition of SARS-CoV-2 infection by and the oral microbiome.

Microbiol Spectr. 2024-10-3

[6]
Identification of receptors and factors associated with human coronaviruses in the oral cavity using single-cell RNA sequencing.

Heliyon. 2024-3-16

[7]
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[8]
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[9]
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[10]
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本文引用的文献

[1]
SARS-CoV-2 infection of the oral cavity and saliva.

Nat Med. 2021-5

[2]
Oral manifestations of COVID-2019-related multisystem inflammatory syndrome in children: a review of 47 pediatric patients.

J Am Dent Assoc. 2021-3

[3]
Association between periodontitis and severity of COVID-19 infection: A case-control study.

J Clin Periodontol. 2021-4

[4]
Association of Viral Infections With Oral Cavity Lesions: Role of SARS-CoV-2 Infection.

Front Med (Lausanne). 2021-1-14

[5]
ACE2 and Furin Expressions in Oral Epithelial Cells Possibly Facilitate COVID-19 Infection via Respiratory and Fecal-Oral Routes.

Front Med (Lausanne). 2020-12-10

[6]
CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells.

Signal Transduct Target Ther. 2020-12-4

[7]
Orofacial manifestations of COVID-19: a brief review of the published literature.

Braz Oral Res. 2020-10-30

[8]
SARS-CoV-2 Detection in Gingival Crevicular Fluid.

J Dent Res. 2021-2

[9]
Cardiorenal Tissues Express SARS-CoV-2 Entry Genes and Basigin (BSG/CD147) Increases With Age in Endothelial Cells.

JACC Basic Transl Sci. 2020-11

[10]
Patient Trajectories Among Persons Hospitalized for COVID-19 : A Cohort Study.

Ann Intern Med. 2021-1

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