The Food Science and Technology College, Shihezi University, Shihezi, Xinjiang 832000, PR China.
The Food Science and Technology College, Shihezi University, Shihezi, Xinjiang 832000, PR China; The Food Science and Technology College, Jiangnan University, Wuxi, Jiangsu 214000, PR China.
Int J Biol Macromol. 2021 Aug 31;185:1036-1049. doi: 10.1016/j.ijbiomac.2021.06.152. Epub 2021 Jun 25.
In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated. Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography. Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→. Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity. Also, it has a good ability to anti-biofilms. Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
在这项研究中，研究了从骆驼奶中分离出的植物乳杆菌 MT41-11 的 EPS 的化学结构和生物活性特性。使用 DEAE-Sepharose 和 Sephadex G-100 色谱法获得了两种分子量约为 69.0 kDa 的多糖级分（EPS-1、EPS-2）。根据单糖组成、FT-IR 和 1D、2D NMR 谱图，得出 EPS-1 的主链由→2)-α-d-Manp-(1→、→3)-α-d-Manp-(1→和含有α-d-Manp-(1→的支链组成，EPS-2 的主链由→6)-β-d-Glcp-(1→组成，并且具有含有→2)-α-l-Fucp-(1→、→3)-α-d-Glcp-(1→、→2)-α-d-Glcp-(1→、β-d-Glcp-(1→和α-d-Glcp-(1→的支链。值得注意的是，体外试验表明，EPS 具有多种生物活性特性，包括刺激乳酸菌生长和具有高 DPPH 自由基清除活性。此外，它还具有良好的抗生物膜能力。总体而言，对所有数据的分析表明，植物乳杆菌 MT41-11 的 EPS 可用作抗氧化剂、抗生物膜剂，并且也是保健食品或医药行业中潜在的益生元候选物。
