用于测定人和牛脂肪组织中半挥发性有机化合物的快速、低成本净化程序。
Rapid, low-cost cleanup procedure for determination of semivolatile organic compounds in human and bovine adipose tissues.
作者信息
Liao W, Smith W D, Chiang T C, Williams L R
机构信息
Lockheed Engineering and Management Services Co., Inc., Environmental Programs, Las Vegas, NV 89119.
出版信息
J Assoc Off Anal Chem. 1988 Jul-Aug;71(4):742-7.
A gas chromatographic/mass spectrometric (GC/MS) method is described for determination of organic environmental pollutants in human and bovine adipose tissues. Compounds such as organochlorine pesticides, polychlorinated biphenyls, polynuclear aromatic hydrocarbons, polychlorinated aromatics, and brominated aromatics are extracted with organic solvents and separated from coextracted lipids on a Florisil column. The eluate is concentrated and compounds are identified and quantitated by GC/MS analysis. The method was evaluated in a single laboratory for ability to recover compounds of environmental and regulatory importance. Except for a few more polar compounds, such as phthalates and phosphates, recoveries averaged about 85%. The elution system maximized recovery and allowed minimal coelution of lipid materials. Detection limits for most compounds studied were in the range of 5-50 ng/g (ppb).
描述了一种气相色谱/质谱（GC/MS）方法，用于测定人体和牛脂肪组织中的有机环境污染物。有机氯农药、多氯联苯、多环芳烃、多氯芳烃和溴化芳烃等化合物用有机溶剂萃取，并在弗罗里硅土柱上与共萃取的脂质分离。洗脱液经浓缩后，通过GC/MS分析对化合物进行鉴定和定量。该方法在单个实验室中进行了评估，以确定其对具有环境和监管重要性的化合物的回收率。除了一些极性更强的化合物，如邻苯二甲酸盐和磷酸盐外，回收率平均约为85%。洗脱系统使回收率最大化，并使脂质材料的共洗脱最少。大多数研究化合物的检测限在5-50 ng/g（ppb）范围内。
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