Laffi R, Marchetti S, Marchetti M
Università di Bologna, Dipartimento di Biochimica, Italy.
J Assoc Off Anal Chem. 1988 Jul-Aug;71(4):826-8.
A simple method is presented for determination of menadione in vitamin premixes and feedstuffs by normal-phase liquid chromatography (LC). Vitamin K3 is extracted and converted to free menadione, which can be determined directly by LC analysis. Peak area or height is measured at 251 nm, and menadione is quantitated by comparison with the working standard. Menadione can be estimated with a detection limit of 2.5 ppm. Recoveries for premixes ranged from 97.3 to 98.3% and for feedstuffs from 93.7 to 96.8%. The method allows the assay of all commercial K3 compounds in pure or stabilized form and is applicable to a wide variety of feeds and premixes.
介绍了一种通过正相液相色谱法（LC）测定维生素预混料和饲料中维生素K3的简单方法。维生素K3被提取并转化为游离甲萘醌，可通过LC分析直接测定。在251nm处测量峰面积或峰高，并通过与工作标准品比较对甲萘醌进行定量。甲萘醌的检测限为2.5ppm。预混料的回收率在97.3%至98.3%之间，饲料的回收率在93.7%至96.8%之间。该方法可测定所有纯品或稳定形式的商业K3化合物，适用于多种饲料和预混料。
