采用荧光检测法，通过液相色谱法测定动物饲料和谷物中的玉米赤霉烯酮和玉米赤霉醇。

Liquid chromatographic determination of zearalenone and zearalenol in animal feeds and grains, using fluorescence detection.

作者信息

Bagneris R W, Gaul J A, Ware G M

出版信息

J Assoc Off Anal Chem. 1986 Sep-Oct;69(5):894-8.

Abstract

A liquid chromatographic (LC) method was developed for the determination of zearalenone and zearalenol in grains and mixed animal feeds. Samples are extracted with chloroform and purified by a base-acid liquid-liquid partition. Zearalenone and zearalenol are separated by reverse phase LC and determined by fluorescence detection, excitation wavelength 236 nm with a 418 nm cutoff filter. The method was applied to the determination of zearalenone and zearalenol in 395 survey samples of corn, oats, barley, sorghum, silage, and finished feeds. The limit of detection is 10 ng/g for both toxins. The range of naturally occurring toxins found was 10-4,000 ng/g. Average recoveries were 84% for zearlenone and 69% for zearalenol. Coefficients of variation were 24.6% for zearalenone and 30.8% for zearalenol for crop year 1980, and 28.3% for zearalenone and 22.0% for zearalenol for crop year 1981.

摘要

建立了一种液相色谱（LC）法，用于测定谷物和混合动物饲料中的玉米赤霉烯酮和玉米赤霉醇。样品用氯仿提取，通过碱-酸液-液分配进行纯化。玉米赤霉烯酮和玉米赤霉醇通过反相液相色谱分离，并用荧光检测法测定，激发波长为236nm，配有418nm截止滤光片。该方法应用于395份玉米、燕麦、大麦、高粱、青贮饲料和成品饲料调查样品中玉米赤霉烯酮和玉米赤霉醇的测定。两种毒素的检测限均为10ng/g。所发现的天然毒素含量范围为10-4000ng/g。玉米赤霉烯酮的平均回收率为84%，玉米赤霉醇的平均回收率为69%。1980年作物年度，玉米赤霉烯酮的变异系数为24.6%，玉米赤霉醇的变异系数为30.8%；1981年作物年度，玉米赤霉烯酮的变异系数为28.3%，玉米赤霉醇的变异系数为22.0%。