Glutathione and its dependent enzymes' modulatory responses to neonicotinoid insecticide sulfoxaflor induced oxidative damage in zebrafish in vivo.

The use of neonicotinoid insecticides has progressively increased worldwide when compared with other insecticide groups. Due to this increase, non-target animal species such as fish are exposed to neonicotinoids from different sources, so they can be accumulated at trophic levels and cause various toxic effects by reaching humans. There are limited studies related to the toxic effects of neonicotinoid sulfoximine insecticides including sulfoxaflor on non-target species. The purpose of the present study was to evaluate the effects of sulfoxaflor on GSH-related antioxidants and to determine oxidative stress-producing effect of sulfoxaflor in the gill of zebrafish ( For this purpose, three sublethal concentrations of sulfoxaflor 0.87 mg/L (2.5% of 96 h LC), 1.75 mg/L (5% of 96 h LC), 3.51 mg/L (10% of 96 h LC) of sulfoxaflor were exposed to zebrafish for 24, 48, and 96 h. GSH related antioxidants were evaluated by analyzing tGSH levels and GPx, GR, GST specific enzyme activities in the gill of zebrafish. The oxidative damage of sulfoxaflor on gill cells was determined by measuring TBARS levels. The results of this study demonstrated that sulfoxaflor activated GSH related antioxidants by increasing tGSH levels, GPx, GR enzyme activities and by diminishing GST enzyme activity in the gill of zebrafish. Sulfoxaflor also caused oxidative damage in the gill of zebrafish by increasing lipid peroxidation. In conclusion, this study indicated that sulfoxaflor led to oxidative stress and activation of GSH related antioxidants in the gill of zebrafish.