Piner Benli Petek, Çelik Mehmet
Department of Veterinary Pharmacology and Toxicology, Faculty of Ceyhan Veterinary Medicine, Cukurova University, Adana 01330, Turkey.
Department of Veterinary Food Hygiene and Technology, Faculty of Ceyhan Veterinary Medicine, Cukurova University, Adana 01330, Turkey.
Toxics. 2021 Apr 1;9(4):73. doi: 10.3390/toxics9040073.
Sulfoxaflor is the first member of the neonicotinoid-sulfoximine insecticides that acts as an agonist of nicotinic acetylcholine receptors (nAChRs). This study investigated the acute effects of sulfoxaflor on acetylcholinesterase (AChE; EC 3.1.1.7) enzyme activity in the brain and muscle tissues of zebrafish () as a model organism. The zebrafish were exposed to 0.87 mg/L (2.5% of 96 h 50% lethal concentration (LC), 1.75 mg/L (5% of 96 h LC) and 3.51 mg/L (10% of 96 h LC) of sulfoxaflor for 24 h-48 h and 96 h periods. AChE enzyme activities were analysed by a spectrophotometric method in the brain and muscle tissues. The results of this study showed that in vivo acute sulfoxaflor exposure significantly increased AChE enzyme activity in the brain and muscle tissues of zebrafish. The induction percentages of AChE were between 10 and 83%, and 19 and 79% for brain and muscle tissues, respectively. As a result, it was found that sulfoxaflor had an effect on AChE enzyme activity in the two main tissues containing this enzyme, and it can be considered as a potential neuroactive compound for zebrafish.
氟啶虫胺腈是新烟碱-磺胺亚胺类杀虫剂的首个成员，它作为烟碱型乙酰胆碱受体（nAChRs）的激动剂发挥作用。本研究以斑马鱼（）作为模式生物，调查了氟啶虫胺腈对其脑和肌肉组织中乙酰胆碱酯酶（AChE；EC 3.1.1.7）酶活性的急性影响。将斑马鱼暴露于0.87毫克/升（96小时半数致死浓度（LC）的2.5%）、1.75毫克/升（96小时LC的5%）和3.51毫克/升（96小时LC的10%）的氟啶虫胺腈中24小时 - 48小时和96小时。采用分光光度法分析脑和肌肉组织中的AChE酶活性。本研究结果表明，体内急性暴露于氟啶虫胺腈会显著增加斑马鱼脑和肌肉组织中的AChE酶活性。脑和肌肉组织中AChE的诱导百分比分别在10%至83%和19%至79%之间。结果发现，氟啶虫胺腈对含有该酶的两个主要组织中的AChE酶活性有影响，并且可以被认为是斑马鱼的一种潜在神经活性化合物。
