使用基因编码钙指示剂研究星形胶质细胞生理学：实用指南。
Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide.
作者信息
Lohr Christian, Beiersdorfer Antonia, Fischer Timo, Hirnet Daniela, Rotermund Natalie, Sauer Jessica, Schulz Kristina, Gee Christine E
机构信息
Division of Neurophysiology, University of Hamburg, Hamburg, Germany.
Institute of Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
出版信息
Front Cell Neurosci. 2021 Jun 11;15:690147. doi: 10.3389/fncel.2021.690147. eCollection 2021.
Ca imaging is the most frequently used technique to study glial cell physiology. While chemical Ca indicators served to visualize and measure changes in glial cell cytosolic Ca concentration for several decades, genetically encoded Ca indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.
钙成像技术是研究胶质细胞生理学最常用的技术。几十年来，化学钙指示剂用于可视化和测量胶质细胞胞质钙浓度的变化，而近年来，基因编码钙指示剂（GECIs）已成为研究的前沿技术。自首个GECI开发以来已取得了重大进展，目前存在大量具有不同物理特性的GECI，因此难以选择最佳的钙指示剂。本综述讨论了一些最常用的GECI及其在胶质细胞研究中的适用性。
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