Karr L J, Van Alstine J M, Snyder R S, Shafer S G, Harris J M
Space Science Laboratory, NASA/Marshall Space Flight Center, Huntsville, AL 35812.
J Chromatogr. 1988 Jun 17;442:219-27. doi: 10.1016/s0021-9673(00)94470-4.
Previous work has shown that polyethylene glycol (PEG)-bound antibodies can be used as affinity ligands in PEG-dextran two-phase systems to provide selective partitioning of cells to the PEG-rich phase. In the present work we show that immunoaffinity partitioning can be simplified by use of PEG-modified Protein A which complexes with unmodified antibody and cells and shifts their partitioning into the PEG-rich phase, thus eliminating the need to prepare a PEG-modified antibody for each cell type. In addition, we provide a more rigorous test of the original technique with PEG-bound antibodies by showing that it is effective at shifting the partitioning of either cell type of a mixture of two cell populations.
先前的研究表明，聚乙二醇（PEG）结合的抗体可作为PEG-葡聚糖双相系统中的亲和配体，使细胞选择性地分配到富含PEG的相中。在本研究中，我们表明，通过使用与未修饰抗体及细胞结合并将它们的分配转移至富含PEG相的PEG修饰的蛋白A，免疫亲和分配可得以简化，从而无需为每种细胞类型制备PEG修饰的抗体。此外，我们通过证明其能有效改变两个细胞群体混合物中任一种细胞类型的分配，对使用PEG结合抗体的原始技术进行了更严格的测试。
