Karr L J, Shafer S G, Harris J M, van Alstine J M, Snyder R S
J Chromatogr. 1986 Feb 28;354:269-82. doi: 10.1016/s0021-9673(01)87028-x.
Poly(ethylene glycol) (PEG) was covalently coupled to IgG antibody preparations directed against human red blood cells. This modification reduces the tendency of the antibody to agglutinate cells and increases its affinity for the upper phase in dextran-PEG aqueous two-phase systems. These effects are related to the molecular weight of the PEG used for modification and to the number of PEG molecules attached to the antibody. Exposure of human red blood cells to PEG-modified antibody causes a substantial and specific increase in cell partition into the PEG-rich phase in a number of PEG-dextran aqueous two-phase systems. Pertinent phase-system parameters were examined. Following a single incubation with PEG-derivatized antibody, a mixture of sheep and human red blood cells was completely separated in 100 min by a 30-transfer countercurrent extraction using a two phase system which normally offers little resolution.
聚乙二醇（PEG）与针对人类红细胞的IgG抗体制剂共价偶联。这种修饰降低了抗体使细胞凝集的倾向，并增加了其在葡聚糖 - PEG水相双相系统中对上相的亲和力。这些效应与用于修饰的PEG的分子量以及连接到抗体上的PEG分子数量有关。在多个PEG - 葡聚糖水相双相系统中，将人类红细胞暴露于PEG修饰的抗体中会导致细胞在富含PEG的相中分配显著且特异性增加。研究了相关的相系统参数。在用PEG衍生化抗体单次孵育后，通过使用通常分辨率较低的双相系统进行30次转移的逆流萃取，在100分钟内将绵羊和人类红细胞的混合物完全分离。
