University of Chinese Academy of Sciences, Beijing 100049, China.
Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
J Agric Food Chem. 2021 Jul 7;69(26):7409-7419. doi: 10.1021/acs.jafc.1c01817. Epub 2021 Jun 28.
An increasing number of strains have been identified, and the removal capacity of zearalenone (ZEN) was determined; however, they failed to reveal the detoxification mechanism and transformation product. Here, Y816, which could transform 40 mg/L of ZEN within 7 h of fermentation, was identified and studied. First, the biotransformation products of ZEN and 17-β-estradiol (E2) were identified as ZEN-14-phosphate and E2-3-phosphate by HPLC-TOF-MS and NMR, respectively. An intracellular zearalenone phosphotransferase (ZPH) was found through transcriptome sequencing analysis of Y816. The phosphorylated reaction conditions of ZEN by ZPH were further revealed in this work. Furthermore, the phosphorylated conjugates showed reduced estrogenic toxicity compared with their original substances (ZEN and α/β-zearalenol) using an engineered yeast biosensor system. The first report on the phosphorylated conjugated mode of ZEN in Y816 will inspire new perspectives on the biotransformation of ZEN in strains.
越来越多的菌株被鉴定出来，并且确定了它们对玉米赤霉烯酮（ZEN）的去除能力；然而，它们未能揭示解毒机制和转化产物。在这里，鉴定并研究了能够在发酵 7 小时内转化 40mg/L ZEN 的 Y816。首先，通过 HPLC-TOF-MS 和 NMR 分别将 ZEN 和 17-β-雌二醇（E2）的生物转化产物鉴定为 ZEN-14-磷酸和 E2-3-磷酸。通过对 Y816 的转录组测序分析，发现了一种细胞内玉米赤霉烯酮磷酸转移酶（ZPH）。本工作进一步揭示了 ZPH 对 ZEN 的磷酸化反应条件。此外，使用工程酵母生物传感器系统，与原始物质（ZEN 和α/β-玉米赤霉醇）相比，磷酸化缀合物显示出降低的雌激素毒性。这是关于 Y816 中 ZEN 磷酸化缀合模式的第一个报告，将为 ZEN 在菌株中的生物转化提供新的视角。
