Department of Mechanical Engineering, Ashesi University, 1 University Avenue, Berekuso, Ghana.
Department of Materials Science and Engineering, African University of Science and Technology, FCT, Abuja, Nigeria.
J Biomed Mater Res A. 2021 Dec;109(12):2611-2624. doi: 10.1002/jbm.a.37254. Epub 2021 Jun 28.
This article presents the results of cell-surface interactions on polydimethylsiloxane (PDMS)-based substrates coated with nanoscale gold (Au) thin films. The surfaces of PDMS and PDMS-magnetite (MNP)-based substrates were treated with UV-ozone, prior to thermal vapor deposition (sputter-coated) of thin films of titanium (Ti) onto the substrates to improve the adhesion of Au coatings. The thin layer of Ti was thermally evaporated to improve interfacial adhesion, which was enhanced by a 40-nm thick film microwrinkled/buckled wavy layer of Au, that was coated to enhance cell-surface interactions and protein absorption. Cell-surface interactions were studied on the hybrid surfaces using a combination of optical and fluorescence microscopy. Consequently, cell proliferation and surface cytotoxicity (of the sputter-coated PDMS surfaces) were elucidated by characterizing the metabolic activity in the presence of breast cancer and normal breast cells. The photothermal conversion efficiency associated with laser-materials interactions with the PDMS/PDMS-magnetite-based composites was shown to have an optimum efficiency of ~31.8%. The implications of the results are discussed for potential applications of PDMS nanocomposites in implantable biomedical devices.
本文介绍了在涂覆纳米金（Au）薄膜的聚二甲基硅氧烷（PDMS）基基底上的细胞表面相互作用的结果。在热蒸发（溅射涂覆）之前，先将 PDMS 和 PDMS-磁铁矿（MNP）基基底的表面用 UV-臭氧处理，以提高 Au 涂层的附着力。在基底上热蒸发一层薄薄的 Ti 以改善界面附着力，然后涂覆 40nm 厚的微波皱/波纹状 Au 薄膜，以增强细胞表面相互作用和蛋白质吸收。使用光学和荧光显微镜的组合研究了混合表面上的细胞表面相互作用。因此，通过在存在乳腺癌和正常乳腺细胞的情况下对代谢活性进行表征，阐明了溅射涂覆的 PDMS 表面的细胞增殖和表面细胞毒性。与激光材料相互作用相关的 PDMS/PDMS-磁铁矿基复合材料的光热转换效率显示出约 31.8%的最佳效率。讨论了这些结果对于 PDMS 纳米复合材料在可植入生物医学设备中的潜在应用的意义。
