通过力谱法对碱基类似物修饰的核酸进行力学特性分析。

Mechanical characterization of base analogue modified nucleic acids by force spectroscopy.

机构信息

Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Sweden.

Small Biosystems Lab, Condensed Matter Physics Department, Universitat de Barcelona, C/Marti i Franques 1, Barcelona 08028, Spain.

出版信息

Phys Chem Chem Phys. 2021 Jul 7;23(26):14151-14155. doi: 10.1039/d1cp01985f.

DOI:10.1039/d1cp01985f

PMID:34180930

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8261857/

Abstract

We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy.

摘要

我们使用带有修饰碱基的单链 DNA 发夹的机械展开来准确评估核酸的分子内和分子间力。正如预期的那样，修饰稳定了杂交发夹，但我们也观察到在展开的发夹中存在有趣的堆积相互作用。我们的研究强调了在力谱学中使用碱基修饰核酸的好处。