Department of Cardiology, Wuhan Red Cross Hospital, Wuhan, 430015, China.
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Curr Med Sci. 2021 Oct;41(5):936-943. doi: 10.1007/s11596-021-2374-6. Epub 2021 Jun 28.
Salidroside is the active ingredient extracted from Rhodiola rosea, and has been reported to show protective effects in cerebral ischemia, but the exact mechanisms of neuronal protective effects are still unrevealed. In this study, the protective effects of salidroside (1 µmol/L) in ameliorating neuronal injuries induced by oxygen-glucose deprivation (OGD), which is a classical model of cerebral ischemia, were clarified. The results showed that after 8 h of OGD, the mouse hippocampal neuronal cell line HT22 cells showed increased cell death, accompanied with mitochondrial fragmentation and augmented mitophagy. However, the cell viability of HT22 cells showed significant restoration after salidroside treatment. Mitochondrial morphology and mitochondrial function were effectively preserved by salidroside treatment. The protective effects of salidroside were further related to the prevention of mitochondrial over-fission. The results showed that mTOR could be recruited to the mitochondria after salidroside treatment, which might be responsible for inhibiting excessive mitophagy caused by OGD. Thus, salidroside was shown to play a protective role in reducing neuronal death under OGD by safeguarding mitochondrial function, which may provide evidence for further translational studies of salidroside in ischemic diseases.
红景天苷是从红景天中提取的有效成分，已被报道具有脑缺血保护作用，但神经元保护的确切机制仍未阐明。在这项研究中，明确了红景天苷（1 μmol/L）对改善氧葡萄糖剥夺（OGD）诱导的神经元损伤的保护作用，OGD 是一种经典的脑缺血模型。结果表明，在 OGD 8 小时后，小鼠海马神经元细胞系 HT22 细胞的细胞死亡增加，伴随着线粒体碎片化和增强的线粒体自噬。然而，红景天苷处理后 HT22 细胞的细胞活力明显恢复。红景天苷处理有效地保留了线粒体形态和线粒体功能。红景天苷的保护作用与预防线粒体过度分裂进一步相关。结果表明，红景天苷处理后 mTOR 可以募集到线粒体，这可能负责抑制 OGD 引起的过度线粒体自噬。因此，红景天苷通过保护线粒体功能在 OGD 下减少神经元死亡中发挥保护作用，这可能为红景天苷在缺血性疾病中的进一步转化研究提供证据。
