Department of Pathology, The Ohio State University, Columbus, Ohio, USA.
Center for Biostatistics, College of Medicine, Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA.
Am J Hypertens. 2021 Nov 20;34(11):1196-1202. doi: 10.1093/ajh/hpab103.
Hypertension is a leading cause of chronic kidney disease worldwide. Early studies demonstrated the short-term effects of hypertension on kidney function and morphology in ablative nephropathy. The aim of this study was to investigate the long-term consequences of hypertension in 5/6 nephrectomy (5/6NE) model.
Reduction of the kidney mass by 5/6NE was created in spontaneous hypertensive rats (SHR) and genetically similar normotensive Wistar Kyoto (WKY) rats. Blood pressure, serum creatinine (SCr), hematuria, and proteinuria were monitored weekly for 23 weeks. Kidney morphology was assessed at the end of the study. Sham-operated rats from both strains were used as controls.
Rats with 5/6NE had increased SCr, blood pressure, hematuria, and proteinuria in both SHR and WKY. Even though the SCr levels and blood pressure were greater in 5/6NE SHR as compared with 5/6NE WKY rats, absolute changes from sham-operated rats were not statistically significant between these 2 groups. 5/6NE SHR had earlier onset and higher proteinuria than 5/6NE WKY rats. Hematuria was similar in 5/6NE SHR and 5/6NE WKY rats. However, 5/6NE SHR had enlarged glomeruli, increased interstitial fibrosis, and prominent intimal thickening in the small arteries/arterioles as compared with 5/6NE WKY rats.
The long-term severity of kidney injury correlated with higher blood pressure. Reduction of the kidney mass increases SCr, hematuria, proteinuria, and blood pressure in both normotensive and hypertensive rats. Histological assessment provides better information about underlying chronic kidney injury than actual changes in SCr and urinalysis.
高血压是全球慢性肾脏病的主要病因。早期研究表明，高血压对消融性肾病中肾功能和形态的短期影响。本研究旨在探讨 5/6 肾切除（5/6NE）模型中高血压的长期后果。
通过 5/6NE 减少自发性高血压大鼠（SHR）和遗传相似的正常血压 Wistar Kyoto（WKY）大鼠的肾脏质量。每周监测血压、血清肌酐（SCr）、血尿和蛋白尿 23 周。研究结束时评估肾脏形态。两种品系的假手术大鼠用作对照。
5/6NE 大鼠的 SCr、血压、血尿和蛋白尿均升高，SHR 和 WKY 大鼠均如此。尽管与 5/6NE WKY 大鼠相比，5/6NE SHR 的 SCr 水平和血压更高，但与假手术大鼠相比，这两组之间的绝对变化并无统计学意义。5/6NE SHR 的蛋白尿发病更早、水平更高，而血尿在 5/6NE SHR 和 5/6NE WKY 大鼠之间相似。然而，与 5/6NE WKY 大鼠相比，5/6NE SHR 的肾小球增大、间质纤维化增加、小动脉/小动脉内膜增厚明显。
肾脏损伤的长期严重程度与更高的血压相关。减少肾脏质量会增加正常血压和高血压大鼠的 SCr、血尿、蛋白尿和血压。组织学评估比实际的 SCr 和尿液分析能提供更好的关于潜在慢性肾脏损伤的信息。
