Ikuma K, Saito Y, Takeda M, Koyama K, Isojima S
Department of Obstetrics and Gynecology, Takarazuka Municipal Hospital, Hyogo.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Jul;40(7):888-94.
The sperm penetration test (SPT) in cervical mucus (CM) is an important test in screening for sperm-CM incompatibility. For a routine SPT, a sufficient amount of preovulatory CM is necessary, but the amount of human CM is often inadequate. We examined estrous bovine CM as a substitute for human CM in the SPT. Preovulatory human CM and estrous bovine CM were collected and stored frozen at -20 degrees C until use. After being thawed at room temperature, the two CM samples were compared as to their pH, spinnbarkeit and ferning patterns, and it was found that they are quite similar. In Kremer's method of SPT, sperm penetration distance, density and motility were similar in human and bovine CM for 12 hours, but thereafter sperm motility became much lower in bovine CM than in human CM. The results of SPT with spermatozoa from patients with oligozoospermia were quite similar in both CMs. When spermatozoa pretreated with antisperm antibody were used for SPT, sperm penetration was completely blocked in human CM, but no inhibition was seen in bovine CM. From these results, we conclude that bovine CM can be substituted for human CM for some, but not all, purposes.
宫颈黏液精子穿透试验（SPT）是筛查精子与宫颈黏液不相容性的一项重要试验。对于常规的SPT，需要足量的排卵前宫颈黏液，但人宫颈黏液的量常常不足。我们研究了用发情期牛的宫颈黏液替代人宫颈黏液进行SPT。收集排卵前的人宫颈黏液和发情期牛的宫颈黏液，于-20℃冷冻保存备用。室温解冻后，比较两种宫颈黏液样本的pH值、拉丝度和羊齿状结晶形态，发现它们非常相似。在克雷默的SPT方法中，人宫颈黏液和牛宫颈黏液中精子的穿透距离、密度和活力在12小时内相似，但此后牛宫颈黏液中精子的活力比人宫颈黏液中的低得多。少精子症患者精子的SPT结果在两种宫颈黏液中非常相似。当使用经抗精子抗体预处理的精子进行SPT时，人宫颈黏液中精子穿透完全受阻，但在牛宫颈黏液中未见抑制现象。从这些结果来看，我们得出结论：牛宫颈黏液可在某些但并非所有目的上替代人宫颈黏液。
