Mangione C M, Medley N E, Menge A C
Int J Fertil. 1981;26(1):20-4.
Estrous bovine cervical mucus (BCM) was evaluated as a practical substitute for midcycle human cervical mucus (HCM) in the sperm-cervical mucus penetration technique (S-CMPT). Analysis of semen samples from 299 men of infertile couples suggested that up to 95% of the S-CMPT analyses with BCM were compatible with results expected with HCM. Results from a secondary S-CMPT with BCM using donor sperm treated with seminal plasma or cervical mucus extracts from partners of infertile couples indicated 70 and 88% agreement with the clinical S-CMPT obtained with the semen and cervical mucus samples, respectively. Seminal plasma samples from men with serum antisperm antibodies decreased the ability of donor sperm to penetrate BCM in the Secondary S-CMPT. Our results indicate that estrous BCM possess those basic qualities of HCM that support human sperm motility, allow for normal sperm penetration, and interact with antibody-treated sperm to inhibit penetration and to produce the sperm shaking phenomenon.
在精子-宫颈黏液穿透技术（S-CMPT）中，对发情期牛宫颈黏液（BCM）作为月经周期中期人宫颈黏液（HCM）的实用替代品进行了评估。对299对不育夫妇男方精液样本的分析表明，高达95%使用BCM的S-CMPT分析结果与使用HCM预期的结果相符。使用来自不育夫妇男方的精浆或宫颈黏液提取物处理的供体精子进行的二次BCM-S-CMPT结果分别表明，与使用精液和宫颈黏液样本获得的临床S-CMPT结果的一致性为70%和88%。患有血清抗精子抗体男性的精浆样本在二次S-CMPT中降低了供体精子穿透BCM的能力。我们的结果表明，发情期BCM具备HCM的那些基本特性，即支持人类精子活力、允许正常精子穿透，并与抗体处理的精子相互作用以抑制穿透并产生精子颤动现象。
