Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair 744103, Andaman and Nicobar Islands, India.
Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair 744103, Andaman and Nicobar Islands, India.
Mar Pollut Bull. 2021 Aug;169:112549. doi: 10.1016/j.marpolbul.2021.112549. Epub 2021 Jun 25.
Deep-sea bacteria when grown in normal environmental conditions get morphologically and genetically adapted to resist the provided culture conditions for their survival, making them a possible aspirant in mercury bioremediation. In this study, seawater samples were collected from different depths of the Central Indian Ocean and seven mercury resistant bacteria (resistant to 100 mg L concentration of inorganic Hg as HgCl) were isolated. Based on 16S rRNA gene sequencing, the identified isolates belong to the genera Pseudomonas, Bacillus and Pseudoalteromonas. The presence of the merA gene in the isolates contributes to the effective volatilization of mercury. The Inductively Coupled Plasma Mass-Spectroscopy analysis revealed that the isolates can reduce up to >80% of inorganic mercury. Moreover, Fourier Transform Infrared spectrum analysis indicates that functional groups play a key role in the mechanism of adaptation towards Hg reduction. Thus, the deep-sea bacteria expressed significant tolerance and reduction potential towards ionic mercury.
深海细菌在正常环境条件下生长时，会在形态和遗传上适应为了生存而提供的培养条件，使它们成为汞生物修复的潜在候选者。在这项研究中，从印度洋中部的不同深度采集了海水样本，并分离出了 7 株耐汞细菌（耐受 100mg/L 浓度的无机 Hg 作为 HgCl）。根据 16S rRNA 基因测序，鉴定出的分离物属于假单胞菌属、芽孢杆菌属和 Pseudalteromonas 属。分离物中 merA 基因的存在有助于汞的有效挥发。电感耦合等离子体质谱分析表明，这些分离物可以将无机汞减少高达>80%。此外，傅里叶变换红外光谱分析表明，功能基团在适应 Hg 还原的机制中起着关键作用。因此，深海细菌对离子态汞表现出显著的耐受性和还原潜力。
