Barreto Matheus C, Braga Raíssa G, Lemos Sherlan G, Fragoso Wallace D
Grupo de Estudos Avançados em Química Analítica, Department of Chemistry, Federal University of Paraíba, João Pessoa, PB 58051-970, Brazil.
Grupo de Estudos Avançados em Química Analítica, Department of Chemistry, Federal University of Paraíba, João Pessoa, PB 58051-970, Brazil.
Food Chem. 2021 Dec 1;364:130407. doi: 10.1016/j.foodchem.2021.130407. Epub 2021 Jun 18.
Melamine is a compound commonly used in the manufacturing of plastic and flame retardant products, but due to its solubility on water and high nitrogen content, it is also used to adulterate milk to mask adulteration by dilution in protein content tests. This work proposes a quick method using excitation-emission matrix (EEM) fluorescence spectroscopy and second-order calibration methods (PARAFAC and U-PLS/RBL) for the identification and quantification of melamine in milk. The proposed method uses a single clean-up step with acetic acid, resulting in a quick, low-cost, and environmentally friendly procedure, in agreement with green chemistry principles. Both PARAFAC and U-PLS/RBL were capable of detecting melamine in milk above 120.6 and 146.5 ppm respectively, adequate for adulterations above 2% in volume, with RMSEPs of 68.6 and 81.9 ppm, respectively.
三聚氰胺是一种常用于制造塑料和阻燃产品的化合物，但由于其在水中的溶解性和高氮含量，它也被用于掺假牛奶，以在蛋白质含量测试中通过稀释来掩盖掺假行为。这项工作提出了一种使用激发-发射矩阵（EEM）荧光光谱法和二阶校准方法（PARAFAC和U-PLS/RBL）来鉴定和定量牛奶中三聚氰胺的快速方法。所提出的方法使用乙酸进行单一净化步骤，从而产生一种快速、低成本且环保的程序，符合绿色化学原则。PARAFAC和U-PLS/RBL都能够分别检测出牛奶中高于120.6 ppm和146.5 ppm的三聚氰胺，适用于体积掺假高于2%的情况，其预测均方根误差分别为68.6 ppm和81.9 ppm。
