Nascimento Carina F, Rocha Diogo L, Rocha Fábio R P
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, P.O. Box 96, 13400-970 Piracicaba, SP, Brazil.
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, P.O. Box 96, 13400-970 Piracicaba, SP, Brazil.
Food Chem. 2015 Feb 15;169:314-9. doi: 10.1016/j.foodchem.2014.07.144. Epub 2014 Aug 7.
An environmental friendly procedure was developed for fast melamine determination as an adulterant of protein content in milk. Triton X-114 was used for sample clean-up and as a fluorophore, whose fluorescence was quenched by the analyte. A linear response was observed from 1.0 to 6.0mgL(-1) melamine, described by the Stern-Volmer equation I°/I=(0.999±0.002)+(0.0165±0.004) CMEL (r=0.999). The detection limit was estimated at 0.8mgL(-1) (95% confidence level), which allows detecting as low as 320μg melamine in 100g of milk. Coefficients of variation (n=8) were estimated at 0.4% and 1.4% with and without melamine, respectively. Recoveries to melamine spiked to milk samples from 95% to 101% and similar slopes of calibration graphs obtained with and without milk indicated the absence of matrix effects. Results for different milk samples agreed with those obtained by high performance liquid chromatography at the 95% confidence level.
开发了一种环境友好的方法，用于快速测定牛奶中作为蛋白质含量掺假物的三聚氰胺。使用Triton X - 114进行样品净化，并将其用作荧光团，其荧光被分析物淬灭。在1.0至6.0mgL(-1)三聚氰胺范围内观察到线性响应，由Stern - Volmer方程I°/I=(0.999±0.002)+(0.0165±0.004) CMEL (r = 0.999)描述。估计检测限为0.8mgL(-1)（95%置信水平），这使得能够检测出100g牛奶中低至320μg的三聚氰胺。变异系数（n = 8）在有和没有三聚氰胺时分别估计为0.4%和1.4%。向牛奶样品中添加三聚氰胺的回收率为95%至101%，并且在有和没有牛奶的情况下获得的校准曲线斜率相似，表明不存在基质效应。不同牛奶样品的结果在95%置信水平下与通过高效液相色谱法获得的结果一致。
