Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China; Scientific Research Center, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Food Chem. 2021 Dec 1;364:130373. doi: 10.1016/j.foodchem.2021.130373. Epub 2021 Jun 16.
A new type of high-density hydrophobic deep eutectic solvents (DESs) were synthesized with nonionic surfactants as hydrogen bond acceptors and hexafluoroisopropanol (HFIP) as hydrogen bond donor. Brij-35 was selected as the optimal nonionic surfactant for the preparation of Brij-35-HFIP-DES (molar ratio 1:20). A vortex-assisted DES-based liquid-liquid microextraction method was proposed for determination of Sudan dyes in tomato chili sauces. The whole pretreatment process only needs 5 min and 1.1 mL of organic solvent. The method with HPLC-DAD shows high efficiency (enrichment factors 89-176 and extraction rates 61.0-74.6%) and good performance with linearity (R ≥ 0.9997) in 0.04-2 μg g range, detection limits of 0.0045-0.0118 μg g, recoveries of 91.6-104.5% and intra-/inter-day precision below 8.0%. A "DES in water in DES" aggregate microstructure was observed in DES-rich phase. The proposed method is simple, quick, eco-friendly, and suits for the efficient extraction and accurate determination of Sudan dyes in tomato chili sauces.
合成了一种新型的高密度疏水性深共晶溶剂（DESs），以非离子表面活性剂作为氢键受体，六氟异丙醇（HFIP）作为氢键供体。Brij-35 被选为制备 Brij-35-HFIP-DES（摩尔比 1:20）的最佳非离子表面活性剂。提出了一种基于涡旋辅助 DES 的液液微萃取方法，用于测定番茄酱中的苏丹染料。整个预处理过程仅需 5 分钟和 1.1 毫升有机溶剂。采用 HPLC-DAD 的方法具有高效性（富集因子为 89-176，萃取率为 61.0-74.6%）和良好的线性性能（R≥0.9997，在 0.04-2μg g 范围内），检测限为 0.0045-0.0118μg g，回收率为 91.6-104.5%，日内和日间精密度低于 8.0%。在 DES 丰富相中观察到了“DES 中的 DES 中的水”聚集微观结构。该方法简单、快速、环保，适用于番茄酱中苏丹染料的高效提取和准确测定。
