Faculty of Food Industry and Agriculture, Department of Food Science & Technology, Standard Research Institute (SRI), Karaj, P.O. Box 31745-139, Iran.
J Chromatogr A. 2019 Apr 26;1591:15-23. doi: 10.1016/j.chroma.2019.01.022. Epub 2019 Jan 9.
A simple, sensitive, efficient and rapid method was developed based on vortex assisted deep eutectic solvent dispersive liquid-liquid microextraction (VA-DES-DLLME) of five commonly used synthetic red dyes (amaranth, ponceau 4R, allura red, azorubine, and erythrosine) in food samples and simultaneous determination by high performance liquid chromatography. The novel hydrophobic deep eutectic solvent was readily prepared by mixing thymol as hydrogen bonding donor and benzyltriethylammonium chloride as hydrogen bonding acceptor at 70 °C for 15 min. Affecting parameters on VA-DES-DLLME were evaluated and optimized. Under optimum conditions, the limits of detection were obtained in the range of 0.01-0.08 μg L and linearity of the dyes were also obtained in the range of 0.5-500 μg L (r ≥ 0.99). The enrichment factors and absolute extraction recoveries were in the range of 95-100 and 90-97, respectively. The relative standard deviations (intra-day and inter-days at 10 and 100 μg L concentration levels) were <6%. Finally, applicability of the proposed method was examined by analyzing the food colors in beverage, jelly and chocolate dragee samples and satisfactory results (the relative recoveries ranged from 94.2 to 100.8%) were obtained.
建立了一种基于涡旋辅助深共晶溶剂分散液液微萃取（VA-DES-DLLME）的简单、灵敏、高效、快速的方法，用于食品样品中五种常用合成红色染料（苋菜红、胭脂红 4R、诱惑红、偶氮玉红和赤藓红）的提取和高效液相色谱同时测定。新型疏水性深共晶溶剂是通过在 70°C 下将麝香草酚作为氢键供体和苄基三乙基氯化铵作为氢键受体混合 15 分钟制备的。评估并优化了 VA-DES-DLLME 的影响参数。在最佳条件下，五种染料的检出限在 0.01-0.08μg/L 范围内，线性范围在 0.5-500μg/L（r≥0.99）范围内。富集因子和绝对萃取回收率分别在 95-100 和 90-97 范围内。相对标准偏差（在 10 和 100μg/L 浓度水平下的日内和日间）<6%。最后，通过分析饮料、果冻和巧克力糖衣样品中的食用色素，考察了该方法的适用性，得到了令人满意的结果（相对回收率在 94.2%至 100.8%之间）。
