State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
J Hazard Mater. 2021 Oct 5;419:126467. doi: 10.1016/j.jhazmat.2021.126467. Epub 2021 Jun 24.
The excess residues of fluoride ions cause serious human health problems, making their detection highly valuable. In this work, a whole-cell-based biosensor was presented for the detection of fluoride ions, which can inhibit the color reaction of 3,3',5,5',-tetramethylbenzidine (TMB) catalyzed by the CotA-laccase of spore surface. This reaction for the detection of fluoride ions could be read out through UV-vis spectrophotometer, smartphone, or standard colorimetric card within 10 min. Under optimum conditions, a linear range of 1-600 μmol L with a detection limit of 0.12 μmol L (3σ/k) was achieved for fluoride ions detection by using UV-vis spectrophotometer. The biosensor coupling with smartphone had a good linear response to fluoride ions concentration in the range of 5-600 μmol L with LOD of 0.90 μmol L (3σ/k). The standard colorimetric card can be directly used for recognizing the fluoride ions level via naked-eyes. A portable kit based on a colorimetric card and smartphone was developed and has been successfully applied for fluoride ions monitoring in surface waters and groundwater. This developed method has several advantages such as rapid, outstanding selectivity and anti-interference, low-cost, ease of operation and storage, and eco-friendliness, meeting the demands of point-of-care testing of fluoride ions and disease prevention.
过量的氟离子残留会导致严重的人类健康问题，因此对其进行检测具有重要的意义。本工作构建了一种基于全细胞的生物传感器，用于检测氟离子。该传感器基于 CotA-漆酶抑制孢子表面 3,3',5,5'-四甲基联苯胺（TMB）显色反应实现对氟离子的检测，通过紫外分光光度计、智能手机或标准比色卡可在 10 分钟内读取检测结果。在优化条件下，紫外分光光度计检测氟离子的线性范围为 1-600 μmol·L，检测限为 0.12 μmol·L（3σ/k）。智能手机与生物传感器联用可实现 5-600 μmol·L 范围内氟离子浓度的线性响应，检测限为 0.90 μmol·L（3σ/k）。标准比色卡可通过肉眼直接识别氟离子水平。基于比色卡和智能手机开发了一种便携式试剂盒，并成功应用于地表水和地下水的氟离子监测。该方法具有快速、选择性好、抗干扰能力强、成本低、操作和储存简便、环保等优点，满足了现场即时检测氟离子和疾病预防的需求。
