Bhat Mahesh P, Kurkuri Mahaveer, Losic Dusan, Kigga Madhuprasad, Altalhi Tariq
Centre for Nano and Material Sciences, Jain University, Jain Global Campus, Bengaluru, 562112, Karnataka, India.
Centre for Nano and Material Sciences, Jain University, Jain Global Campus, Bengaluru, 562112, Karnataka, India.
Anal Chim Acta. 2021 May 15;1159:338439. doi: 10.1016/j.aca.2021.338439. Epub 2021 Mar 23.
A PDMS (Polydimethylsiloxane) microfluidic channel coupled with UV-vis fibre-optic spectrometer and new synthesized colorimetric probe was integrated into an optofluidic based Lab-on-a-chip device for highly sensitive and real-time quantitative measurements of fluoride ions (F¯). An 'S' shaped microchannel in a microfluidic device was designed to act as microreactor to facilitate the continuous reaction between synthetized colorimetric probe (sensor) and F¯ ions. Following this reaction, the UV-vis optical probe in the downstream detection zone of the microfluidic device was used to capture their spectrum and present as F¯ concentration in real-time conditions. An initial study of the developed colorimetric probe with multi-colour change with several binding and chromophore groups such as -OH, -NH and -NO groups confirmed its high sensitivity and selectivity for F¯ ions with a detection limit of 0.79 ppm. The performance of the developed optofluidic device was evaluated for the selective, sensitive detection of F¯ ions including real samples out-performing conventional methods. The technology has advantages such as low sample consumption, rapid analysis, high sensitivity and portability. Presented new Lab-on-a-chip device provides many competitive advantages for the real-time analysis of F¯ ions needed across broad sectors.
一个与紫外可见光纤光谱仪耦合的聚二甲基硅氧烷（PDMS）微流控通道以及新合成的比色探针被集成到一个基于光流体的芯片实验室装置中，用于对氟离子（F¯）进行高灵敏度实时定量测量。微流控装置中的一个“S”形微通道被设计用作微反应器，以促进合成的比色探针（传感器）与F¯离子之间的连续反应。该反应之后，微流控装置下游检测区的紫外可见光学探针用于捕获其光谱，并实时呈现为F¯浓度。对所开发的具有多种结合和发色团基团（如 -OH、-NH 和 -NO 基团）的多色变化比色探针的初步研究证实了其对F¯离子的高灵敏度和选择性，检测限为0.79 ppm。所开发的光流体装置的性能针对F¯离子的选择性、灵敏检测进行了评估，包括实际样品，其性能优于传统方法。该技术具有样品消耗低、分析快速、灵敏度高和便携等优点。所展示的新型芯片实验室装置为广泛领域所需的F¯离子实时分析提供了许多竞争优势。
