Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes, USR3579, Observatoire Océanologique, 66650 Banyuls-sur-mer, France; Division of Biological and Environmental Sciences, Faculty of Natural Sciences, Stirling University, United Kingdom.
Division of Biological and Environmental Sciences, Faculty of Natural Sciences, Stirling University, United Kingdom.
Sci Total Environ. 2021 Nov 1;793:148431. doi: 10.1016/j.scitotenv.2021.148431. Epub 2021 Jun 18.
The use of Benzophenone-3 (BP3), also known as oxybenzone, a common UV filter, is a growing environmental concern in regard to its toxicity on aquatic organisms. Our previous work stressed that BP3 is toxic to Epibacterium mobile, an environmentally relevant marine α-proteobacterium. In this study, we implemented a label-free quantitative proteomics workflow to decipher the effects of BP3 on the E. mobile proteome. Furthermore, the effect of DMSO, one of the most common solvents used to vehicle low concentrations of lipophilic chemicals, was assessed to emphasize the importance of limiting solvent concentration in ecotoxicological studies. Data-independent analysis proteomics highlighted that BP3 induced changes in the regulation of 56 proteins involved in xenobiotic export, detoxification, oxidative stress response, motility, and fatty acid, iron and amino acid metabolisms. Our results also outlined that the use of DMSO at 0.046% caused regulation changes in proteins related to transport, iron uptake and metabolism, and housekeeping functions, underlining the need to reduce the concentration of solvents in ecotoxicological studies.
苯并三唑-3（BP3），又称氧苯酮，是一种常见的紫外线吸收剂，其对水生生物的毒性引起了人们对环境的关注。我们之前的工作强调了 BP3 对具有环境相关性的海洋 α-变形菌移动球菌（Epibacterium mobile）具有毒性。在这项研究中，我们采用无标记定量蛋白质组学工作流程来解析 BP3 对 E. mobile 蛋白质组的影响。此外，还评估了二甲基亚砜（DMSO）的作用，DMSO 是用于运输低浓度亲脂性化学物质的最常用溶剂之一，以强调在生态毒理学研究中限制溶剂浓度的重要性。数据非依赖性分析蛋白质组学强调，BP3 诱导了与外排、解毒、氧化应激反应、运动以及脂肪酸、铁和氨基酸代谢相关的 56 种蛋白质的调节变化。我们的研究结果还表明，在 0.046% DMSO 的作用下，与运输、铁摄取和代谢以及维持细胞功能相关的蛋白质的调节发生变化，这强调了在生态毒理学研究中需要降低溶剂浓度的必要性。
