高脂饮食诱导的动脉粥样硬化在小型猪模型中的高病理学重现性。 克隆技术。
High Pathological Reproducibility of Diet-induced Atherosclerosis in Microminipigs Cloning Technology.
机构信息
Swine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Kikugawa, Japan;
Department of Hygiene and Health Promotion Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
出版信息
In Vivo. 2021 Jul-Aug;35(4):2025-2033. doi: 10.21873/invivo.12471.
BACKGROUND/AIM: The reproducibility of athero - sclerotic lesions was evaluated after the production of cloned-microminipigs and their offspring.
MATERIALS AND METHODS
Cloned-microminipig-parents were produced by microminipigsomatic cell nuclei. These parents were crossbred and delivered males (F1-offspring) were divided into two groups: normal chow diet (NcD)-fed and high-fat/high-cholesterol diet (HcD)-fed groups. One of the F1-offsprings was subjected to cloning, and delivered males (F1-clones) were fed with HcD. After 8 weeks, all animals were necropsied for patho - physiological studies compared to non-cloned-microminipigs.
RESULTS
HcD-fed F1-offspring and F1-clones, but not NcD-fed F1-offspring, exhibited increased serum lipid levels and systemic atherosclerosis, which were comparable to those of HcD-fed non-cloned-microminipigs. Homogeneity of variance analysis demonstrated that standard deviation values of serum lipoprotein and aortic atherosclerosis area from HcD-fed animals decreased in F1-offspring and F1-clones.
CONCLUSION
HcD-induced atherogenesis was highly reproducible in F1-offsprings and F1-clones, indicating that the atherosclerosis-prone genomic background was preserved in the cloned-microminipigs, which can be used for studies on human atherosclerosis and related diseases.
背景/目的：评估了克隆微小猪及其后代粥样硬化病变的可重复性。
材料和方法
通过微小猪体细胞核产生了克隆微小猪父母。这些父母进行了杂交，产下的雄性（F1 后代）被分为两组：正常饲料喂养组（NcD）和高脂肪/高胆固醇饮食喂养组（HcD）。其中一只 F1 后代进行了克隆，产下的雄性（F1 克隆）用 HcD 喂养。8 周后，所有动物均进行尸检，与未克隆的微小猪进行病理生理研究。
结果
HcD 喂养的 F1 后代和 F1 克隆，而非 NcD 喂养的 F1 后代，表现出血清脂质水平升高和全身性动脉粥样硬化，与 HcD 喂养的未克隆微小猪相当。方差齐性分析表明，HcD 喂养动物的血清脂蛋白和主动脉粥样硬化面积的标准差值在 F1 后代和 F1 克隆中降低。
结论
HcD 诱导的动脉粥样硬化发生在 F1 后代和 F1 克隆中具有高度可重复性，表明易患动脉粥样硬化的基因组背景在克隆微小猪中得以保留，可用于研究人类动脉粥样硬化及相关疾病。
Zotero 插件