Swine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Kikugawa, Japan;
Department of Hygiene and Health Promotion Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
In Vivo. 2021 Jul-Aug;35(4):2025-2033. doi: 10.21873/invivo.12471.
BACKGROUND/AIM: The reproducibility of athero - sclerotic lesions was evaluated after the production of cloned-microminipigs and their offspring.
Cloned-microminipig-parents were produced by microminipigsomatic cell nuclei. These parents were crossbred and delivered males (F1-offspring) were divided into two groups: normal chow diet (NcD)-fed and high-fat/high-cholesterol diet (HcD)-fed groups. One of the F1-offsprings was subjected to cloning, and delivered males (F1-clones) were fed with HcD. After 8 weeks, all animals were necropsied for patho - physiological studies compared to non-cloned-microminipigs.
HcD-fed F1-offspring and F1-clones, but not NcD-fed F1-offspring, exhibited increased serum lipid levels and systemic atherosclerosis, which were comparable to those of HcD-fed non-cloned-microminipigs. Homogeneity of variance analysis demonstrated that standard deviation values of serum lipoprotein and aortic atherosclerosis area from HcD-fed animals decreased in F1-offspring and F1-clones.
HcD-induced atherogenesis was highly reproducible in F1-offsprings and F1-clones, indicating that the atherosclerosis-prone genomic background was preserved in the cloned-microminipigs, which can be used for studies on human atherosclerosis and related diseases.
背景/目的：评估了克隆微小猪及其后代粥样硬化病变的可重复性。
通过微小猪体细胞核产生了克隆微小猪父母。这些父母进行了杂交，产下的雄性（F1 后代）被分为两组：正常饲料喂养组（NcD）和高脂肪/高胆固醇饮食喂养组（HcD）。其中一只 F1 后代进行了克隆，产下的雄性（F1 克隆）用 HcD 喂养。8 周后，所有动物均进行尸检，与未克隆的微小猪进行病理生理研究。
HcD 喂养的 F1 后代和 F1 克隆，而非 NcD 喂养的 F1 后代，表现出血清脂质水平升高和全身性动脉粥样硬化，与 HcD 喂养的未克隆微小猪相当。方差齐性分析表明，HcD 喂养动物的血清脂蛋白和主动脉粥样硬化面积的标准差值在 F1 后代和 F1 克隆中降低。
HcD 诱导的动脉粥样硬化发生在 F1 后代和 F1 克隆中具有高度可重复性，表明易患动脉粥样硬化的基因组背景在克隆微小猪中得以保留，可用于研究人类动脉粥样硬化及相关疾病。
