Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
In Vivo. 2021 Jul-Aug;35(4):2035-2040. doi: 10.21873/invivo.12472.
BACKGROUND/AIM: With the demographic change and associated chronic bone loss, the need for cytocompatible bone replacement materials arise in modern medicine. The aim of this in vitro study was to investigate the cytocompatibility of eleven different bone substitute materials and membranes.
Seven bone substitute materials and four membranes were assessed in vitro. The specimens were tested based on their interaction with MC3T3 pre-osteoblasts, through the utilization of viability, proliferation, and cytotoxicity assays. Cell vitality was evaluated using live-dead staining.
Although we found minor differences in cytocompatibility among the assessed materials, all tested materials can be considered as cytocompatible with a viability of more than 70% of the negative control, which indicates the non-toxic range as defined in current, international standards (DIN EN ISO 10993-5:2009, German Institute for Standardization, Berlin, Germany). Direct live-dead staining assays confirmed satisfactory cytocompatibility of all tested membranes.
All examined bone substitute materials and membranes were found to be cytocompatible. In order to assess whether the observed minor differences can impact regenerative processes, further in vivo studies need to be conducted.
背景/目的：随着人口结构的变化和相关的慢性骨丢失，现代医学对细胞相容性骨替代材料的需求日益增加。本体外研究的目的是研究十一种不同的骨替代材料和膜的细胞相容性。
在体外评估了七种骨替代材料和四种膜。通过利用活/死染色评估细胞活力，通过对 MC3T3 前成骨细胞的相互作用，通过使用活力、增殖和细胞毒性测定来测试标本。
尽管我们发现评估材料之间的细胞相容性存在微小差异，但所有测试材料的活力均超过 70%，这表明符合当前国际标准（DIN EN ISO 10993-5:2009，德国标准化协会，柏林，德国）中定义的无毒范围。直接活/死染色测定证实所有测试膜均具有良好的细胞相容性。
所有检查的骨替代材料和膜均被认为具有细胞相容性。为了评估观察到的微小差异是否会影响再生过程，需要进行进一步的体内研究。
