Department of Medical Biology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey;
Regenerative and Restorative Medicine Research Center (REMER), Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul, Turkey.
In Vivo. 2021 Jul-Aug;35(4):2127-2134. doi: 10.21873/invivo.12482.
BACKGROUND/AIM: Mesenchymal stem cells (MSCs) have been widely used for yielding neurons in culture to study nervous system pathologies and develop regenerative approaches. In this study, cellular rearrangements of human MSCs related to the expression of the fibronectin common receptor integrin α5β1 and its cell surface localization during neuronal differentiation, were examined. MATERIALS AND METHODS: Proliferation kinetics of neuronal induced hMSCs (hMd-Neurons) were quantified by BrdU assay, and hMd-Neurons were immunostained for neuronal marker expression. Additionally, cDNA and protein samples were collected at different time points for integrin α5β1 expression analysis. RESULTS: Endogenous integrin α5β1 expression was significantly upregulated by day 6 and maintained until day 12. Cell surface localization of α5β1 integrin was increased by day 6; the integrin was internalized into the cytosol by day 12. CONCLUSION: Integrin dynamics around day 6 of differentiation might be involved in neuronal differentiation and maturation or specification of hMd-Neurons.
背景/目的：间充质干细胞（MSCs）已被广泛用于在培养中产生神经元，以研究神经系统疾病并开发再生方法。在这项研究中，研究了人 MSCs 在神经元分化过程中与纤维连接蛋白共同受体整合素 α5β1 的表达及其细胞表面定位相关的细胞重排。 材料和方法：通过 BrdU 测定法量化诱导神经元的人 MSCs（hMd-Neurons）的增殖动力学，并对 hMd-Neurons 进行神经元标记物表达的免疫染色。此外，还在不同时间点收集 cDNA 和蛋白质样品以分析整合素 α5β1 的表达。 结果：内源性整合素 α5β1 的表达在第 6 天显著上调，并持续到第 12 天。α5β1 整合素的细胞表面定位在第 6 天增加；该整合素在第 12 天被内化到细胞质中。 结论：分化后第 6 天左右整合素的动态变化可能参与了 hMd-Neurons 的神经元分化和成熟或特化。
J Cell Sci. 2003-1-15
Nat Rev Drug Discov. 2020-4-6
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