Department of Anesthesiology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Department of Anesthesiology, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, China.
Aging (Albany NY). 2021 Jun 28;13(12):16733-16748. doi: 10.18632/aging.203193.
Multiple exposures to anesthesia may increase the risk of cognitive impairment in young children. However, the mechanisms underlying this neurodevelopmental disorder remain elusive. In this study, we investigated alteration of the gut microbiota after multiple neonatal exposures to the anesthetic sevoflurane and the potential role of microbiota alteration on cognitive impairment using a young mice model. Multiple neonatal sevoflurane exposures resulted in obvious cognitive impairment symptoms and altered gut microbiota composition. Fecal transplantation experiments confirmed that alteration of the microbiota was responsible for the cognitive disorders in young mice. Microbiota profiling analysis identified microbial taxa that showed consistent differential abundance before and after fecal microbiota transplantation. Several of the differentially abundant taxa are associated with memory and/or health of the host, such as species of Streptococcus, Lachnospiraceae, and Pseudoflavonifractor. The results reveal that abnormal composition of the gut microbiota is a risk factor for cognitive impairment in young mice after multiple neonatal exposures to sevoflurane and provide insight into a potential therapeutic strategy for sevoflurane-related neurotoxicity.
多次接触麻醉可能会增加幼儿认知障碍的风险。然而，这种神经发育障碍的潜在机制仍难以捉摸。在这项研究中，我们使用年幼小鼠模型研究了多次新生期接触麻醉剂七氟醚后肠道微生物群的改变，以及微生物群改变对认知障碍的潜在作用。多次新生期七氟醚暴露导致明显的认知障碍症状和肠道微生物群组成的改变。粪便移植实验证实，微生物群的改变是导致年轻小鼠认知障碍的原因。微生物组分析鉴定出在粪便微生物群移植前后具有一致差异丰度的微生物类群。一些差异丰度的分类群与宿主的记忆和/或健康有关，例如链球菌属、lachnospiraceae 和 pseudoflavonifractor 等物种。研究结果表明，多次新生期接触七氟醚后肠道微生物群的异常组成是年轻小鼠认知障碍的一个危险因素，并为七氟醚相关神经毒性的潜在治疗策略提供了线索。
