Innate Immunity Group, Hospital La Paz Institute for Health Research, La Paz University Hospital, Madrid, Spain.
Tumor Immunology Laboratory, Hospital La Paz Institute for Health Research, La Paz University Hospital, Madrid, Spain.
J Immunol. 2021 Jul 1;207(1):162-174. doi: 10.4049/jimmunol.2001449. Epub 2021 Jun 28.
According to a large number of reported cohorts, sepsis has been observed in nearly all deceased patients with COVID-19. We and others have described sepsis, among other pathologies, to be an endotoxin tolerance (ET)-related disease. In this study, we demonstrate that the culture of human blood cells from healthy volunteers in the presence of SARS-CoV-2 proteins induced ET hallmarks, including impairment of proinflammatory cytokine production, low MHC class II (HLA-DR) expression, poor T cell proliferation, and enhancing of both phagocytosis and tissue remodeling. Moreover, we report the presence of SARS-CoV-2 blood circulating proteins in patients with COVID-19 and how these levels correlate with an ET status, the viral RNA presence of SARS-CoV-2 in plasma, as well as with an increase in the proportion of patients with secondary infections.
根据大量报道的队列研究，几乎所有 COVID-19 死亡患者都观察到了脓毒症。我们和其他人已经描述了脓毒症，以及其他病理学，是一种内毒素耐受（ET）相关的疾病。在这项研究中，我们证明了在 SARS-CoV-2 蛋白存在的情况下培养来自健康志愿者的人血细胞会诱导 ET 特征，包括促炎细胞因子产生受损、低 MHC Ⅱ类（HLA-DR）表达、T 细胞增殖不良，以及吞噬作用和组织重塑增强。此外，我们报告了 COVID-19 患者血液中存在 SARS-CoV-2 循环蛋白，以及这些水平如何与 ET 状态、血浆中 SARS-CoV-2 的病毒 RNA 存在以及继发性感染患者比例增加相关。
