Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE) , Hamburg, Germany.
Institute of Forensic Medicine, University Medical Center Hamburg-Eppendorf (UKE) , Hamburg, Germany.
Ocul Immunol Inflamm. 2020 Jul 3;28(5):721-725. doi: 10.1080/09273948.2020.1770301. Epub 2020 May 29.
To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19).
Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater™. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2.
In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization.
Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.
报道在确诊的 2019 年新型冠状病毒病（COVID-19）死亡患者的人视网膜中存在严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的病毒核糖核酸（RNA）。
在尸检期间，从 14 名确诊 COVID-19 疾病的死亡患者中取出 14 只眼睛。采集人视网膜样本并用 RNAlater™固定。进行实时逆转录-聚合酶链反应（RT-PCR）以检测 SARS-CoV-2 的三种不同病毒 RNA 序列（RdRp 基因、E 基因和 Orf1 基因）。
在 14 只眼中的 3 只眼中，在 COVID-19 死亡患者的视网膜中检测到了 SARS-CoV-2 病毒 RNA。由于 RT-PCR 分析显示三种不同序列（RdRp 基因、E 基因和 Orf1 基因）的阳性结果，根据世界卫生组织的标准，证明了 SARS-CoV-2 病毒 RNA 存在于人视网膜中。
在 COVID-19 患者的视网膜中可检测到 SARS-CoV-2 的病毒 RNA。
