State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China.
The Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Chinese Academy of Sciences, Wuhan, People's Republic of China.
J Immunol. 2021 Jul 1;207(1):244-256. doi: 10.4049/jimmunol.2000891. Epub 2021 Jun 28.
Ovarian tumor domain-containing 6B () belongs to the OTU deubiquitylating enzyme family. In this study, we report that zebrafish is induced upon viral infection, and overexpression of suppresses cellular antiviral response. Disruption of in zebrafish increases the survival rate upon spring viremia of carp virus and grass carp reovirus exposure. Further assays indicate that otud6b interacts with irf3 and irf7 and diminishes traf6-mediated K63-linked polyubiquitination of irf3 and irf7. In addition, the OTU domain is required for to repress IFN-1 activation and K63-linked polyubiquitination of irf3 and irf7. Moreover, also attenuates tbk1 to bind to irf3 and irf7, resulting in the impairment of irf3 and irf7 phosphorylation. This study provides, to our knowledge, novel insights into function and sheds new lights on the regulation of and by deubiquitination in IFN-1 signaling.
卵巢肿瘤结构域包含 6B () 属于 OTU 去泛素化酶家族。在这项研究中，我们报告说，斑马鱼 在病毒感染时被诱导，并且 的过表达抑制细胞抗病毒反应。斑马鱼 中的破坏增加了在锦鲤病毒和草鱼虹彩病毒暴露时的存活率。进一步的测定表明，otud6b 与 irf3 和 irf7 相互作用，并减少 traf6 介导的 irf3 和 irf7 的 K63 连接多泛素化。此外，OTU 结构域对于 抑制 IFN-1 激活和 irf3 和 irf7 的 K63 连接多泛素化是必需的。此外， 还减弱了 tbk1 与 irf3 和 irf7 的结合，导致 irf3 和 irf7 的磷酸化受损。这项研究提供了我们所知的 功能的新见解，并阐明了 及其 通过 IFN-1 信号转导的去泛素化的调节。
