斑马鱼通过非酶活性负调控 irf3 和 irf7 的磷酸化来抑制抗病毒反应。
Zebrafish Negatively Regulates Antiviral Responses by Attenuating Phosphorylation of irf3 and irf7 Independent of Its Enzymatic Activity.
机构信息
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China.
Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan, People's Republic of China.
出版信息
J Immunol. 2021 Dec 15;207(12):3050-3059. doi: 10.4049/jimmunol.2100318. Epub 2021 Nov 19.
is one member of the sirtuin family proteins with NAD (NAD)-dependent histone deacetylase activity. In this study, we report that zebrafish is induced upon viral infection, and overexpression of suppresses cellular antiviral responses. Disruption of in zebrafish increases the survival rate upon spring viremia of carp virus infection. Further assays indicate that sirt7 interacts with irf3 and irf7 and attenuates phosphorylation of irf3 and irf7 by preventing tbk1 binding to irf3 and irf7. In addition, the enzymatic activity of sirt7 is not required for to repress IFN-1 activation. To our knowledge, this study provides novel insights into function and sheds new light on the regulation of and by attenuating phosphorylation.
是 sirtuin 家族蛋白的成员之一，具有 NAD（烟酰胺腺嘌呤二核苷酸）依赖性组蛋白去乙酰化酶活性。在这项研究中，我们报告了斑马鱼在病毒感染时被诱导，并且过表达会抑制细胞抗病毒反应。在斑马鱼中破坏会增加鲤鱼病毒血症 Spring 病毒感染时的存活率。进一步的实验表明，sirt7 与 irf3 和 irf7 相互作用，并通过防止 tbk1 与 irf3 和 irf7 结合来减弱 irf3 和 irf7 的磷酸化，从而抑制 IFN-1 的激活。此外，sirt7 的酶活性对于抑制 IFN-1 激活并不是必需的。据我们所知，这项研究为提供了关于功能的新见解，并阐明了通过减弱磷酸化来调节和的新机制。
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