Department of Experimental Hematooncology, Medical University, Lublin, Poland.
Department of Medical Biology, Institute of Rural Health, Lublin, Poland.
Ann Agric Environ Med. 2021 Jun 14;28(2):291-299. doi: 10.26444/aaem/133801. Epub 2021 Mar 11.
Due to the fact that lymphocytes NK (natural killer cells) are the first line of defence of the body against cancer, one of the goals of modern immunotherapy is the enhancement of their natural activities for the effective recognition, detection, and elimination of cancer cells.
The aim of the study was to evaluate the influence of selected phytochemicals (curcumin and resveratrol) and plant extracts (chlorella and goji berries) on NK cells viability and proliferation, as well as cytotoxic activity against colon cancer - one of the most common cancer worldwide.
The impact of phytochemicals, viability and proliferation of plant extracts on NK cells was examined in NK-92 cells using both LDH and MTT assays. The immunomodulatory properties of selected compounds were tested against human colon cancer cell line LS180 using the MTT test.
Extracts of chlorella and goji berries significantly increased NK cell proliferation, while curcumin and resveratrol did not affect this process. Curcumin, as well as extracts of chlorella and goji berries, did not impact NK viability, while resveratrol significantly increased it. LDH test revealed the cytotoxic effect of chlorella extract and curcumin in NK-92 cell cultures. On the contrary, goji berries extract significantly decreased LDH level, while resveratrol did not affect the integrity of NK cell membranes. Studies conducted in co-cultures NK cells, also directly eliminated colon cancer cells.
Performed studies revealed immunomodulatory properties of goji berries extract, which improved viability and proliferation of NK cells, and above all, significantly increased their ability to recognize and eliminate colon cancer cells.
由于自然杀伤细胞（NK）是人体抵抗癌症的第一道防线，因此现代免疫疗法的目标之一是增强其自然活性，以有效识别、检测和消除癌细胞。
本研究旨在评估选定的植物化学物质（姜黄素和白藜芦醇）和植物提取物（小球藻和枸杞）对 NK 细胞活力和增殖的影响，以及对结直肠癌的细胞毒性作用，结直肠癌是全球最常见的癌症之一。
使用 LDH 和 MTT 测定法，在 NK-92 细胞中研究了植物化学物质、植物提取物对 NK 细胞活力和增殖的影响。使用 MTT 试验测试了选定化合物对人结直肠癌细胞系 LS180 的免疫调节特性。
小球藻和枸杞提取物显著增加了 NK 细胞的增殖，而姜黄素和白藜芦醇则不影响这一过程。姜黄素以及小球藻和枸杞提取物均不影响 NK 细胞活力，而白藜芦醇则显著增加了 NK 细胞活力。LDH 试验显示小球藻提取物和姜黄素在 NK-92 细胞培养物中具有细胞毒性作用。相反，枸杞提取物显著降低了 LDH 水平，而白藜芦醇则不影响 NK 细胞膜的完整性。在 NK 细胞共培养物中进行的研究也直接消除了结肠癌细胞。
本研究揭示了枸杞提取物的免疫调节特性，它提高了 NK 细胞的活力和增殖，最重要的是，显著提高了它们识别和消除结肠癌细胞的能力。
