School of Life Sciences, Shandong University of Technology, Zibo 255049, China.
Laboratory of Developmental and Evolutionary Biology, Shandong University of Technology, Zibo 255049, China.
Biol Open. 2021 Jun 15;10(6). doi: 10.1242/bio.058538. Epub 2021 Jun 29.
MicroRNAs (miRNAs) are a class of evolutionarily conserved small non-coding RNAs that regulate gene expression at the translation level in cell growth, proliferation and differentiation. In addition, some types of miRNAs have been proven to be key modulators of both CNS development and plasticity, such as let-7, miR-9 and miR-124. In this research, we found miR-8b acts as an important regulator involved in brain and eyespot regeneration in Dugesia japonica. miR-8b was highly conserved among species and was abundantly expressed in central nervous system. Here, we detected the expression dynamics of miR-8b by qPCR during the head regeneration of D. japonica. Knockdown miR-8b by anti-MIRs method caused severe defects of eyes and CNS. Our study revealed the evolutionary conserved role of miR-8b in the planarian regeneration process, and further provided more research ideas and available information for planarian miRNAs.
微小 RNA（miRNAs）是一类进化上保守的小非编码 RNA，可在细胞生长、增殖和分化过程中在翻译水平上调节基因表达。此外，一些类型的 miRNAs 已被证明是中枢神经系统发育和可塑性的关键调节剂，如 let-7、miR-9 和 miR-124。在这项研究中，我们发现 miR-8b 在日本三角涡虫的脑和眼点再生中作为一个重要的调节因子发挥作用。miR-8b 在物种间高度保守，在中枢神经系统中大量表达。在这里，我们通过 qPCR 检测了 miR-8b 在日本三角涡虫头部再生过程中的表达动态。用反义 miR 方法敲低 miR-8b 会导致眼睛和中枢神经系统严重缺陷。我们的研究揭示了 miR-8b 在扁形动物再生过程中的进化保守作用，并为扁形动物 miRNAs 提供了更多的研究思路和可用信息。
