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三种新型冠状病毒2型血清学化学发光免疫分析方法的比较及抗体指数与疾病严重程度的临床意义

Comparison of three serological chemiluminescence immunoassays for SARS-CoV-2, and clinical significance of antibody index with disease severity.

作者信息

Lee Nuri, Jeong Seri, Park Min-Jeong, Song Wonkeun

机构信息

Department of Laboratory Medicine, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, South Korea.

出版信息

PLoS One. 2021 Jun 29;16(6):e0253889. doi: 10.1371/journal.pone.0253889. eCollection 2021.

DOI:10.1371/journal.pone.0253889
PMID:34185813
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8241106/
Abstract

BACKGROUND

The clinical significance of the quantitative value of antibodies in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains mostly unidentified. We investigated the dynamics and clinical implications of the SARS-CoV-2 antibody over time using three automated chemiluminescence immunoassays targeting either nucleocapsids or spikes.

METHODS

A total of 126 specimens were collected from 23 patients with confirmed and indeterminate COVID-19 identified by molecular tests. SARS-CoV-2 antibody index was measured using SARS-CoV2 IgG reagent from Alinity (Abbott) and Access (Beckman Coulter) and SARS-CoV2 Total (IgG + IgM) from Atellica (Siemens).

RESULTS

Three immunoassays showed strong correlations with each other (range of Pearson' s correlation coefficient (r) = 0.700-0.854, P < 0.001). Eleven (8.7%) specimens showed inconsistencies. SARS-CoV-2 IgG showed a statistically significantly higher value in patients with severe disease than that in non-severe disease patients (P < 0.001) and was significantly associated with clinical markers of disease severity.

CONCLUSION

The quantitative value of the SARS-CoV-2 IgG antibody measured using automated immunoassays is a significant indicator of clinical severity in patients with COVID-19.

摘要

背景

严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染中抗体定量值的临床意义大多仍不明确。我们使用三种针对核衣壳或刺突的自动化化学发光免疫分析法，研究了SARS-CoV-2抗体随时间的动态变化及其临床意义。

方法

从23例经分子检测确诊和不确定的COVID-19患者中总共收集了126份样本。使用雅培公司Alinity、贝克曼库尔特公司Access的SARS-CoV2 IgG试剂以及西门子公司Atellica的SARS-CoV2 Total（IgG + IgM）试剂测量SARS-CoV-2抗体指数。

结果

三种免疫分析法相互之间显示出很强的相关性（皮尔逊相关系数（r）范围 = 0.700 - 0.854，P < 0.001）。11份（8.7%）样本显示结果不一致。SARS-CoV-2 IgG在重症患者中的值在统计学上显著高于非重症患者（P < 0.001），并且与疾病严重程度的临床指标显著相关。

结论

使用自动化免疫分析法测量的SARS-CoV-2 IgG抗体定量值是COVID-19患者临床严重程度的重要指标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/558e/8241106/87f9413bb0b1/pone.0253889.g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/558e/8241106/87f9413bb0b1/pone.0253889.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/558e/8241106/4914d0f2a261/pone.0253889.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/558e/8241106/2581d58d1283/pone.0253889.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/558e/8241106/d0515f6ed7b4/pone.0253889.g003.jpg
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