Department of Radiation Oncology, University of California San Francisco, Helen Diller Cancer Research Building, 1450 3rd street, HD403, San Francisco, CA 94158, USA.
Department of Radiation Oncology, University of California San Francisco, Precision Cancer Medicine Building, 1825 4th Street, Suite L1101, San Francisco, CA 94158, USA.
Hematol Oncol Clin North Am. 2021 Oct;35(5):963-971. doi: 10.1016/j.hoc.2021.05.007. Epub 2021 Jun 27.
Nasopharyngeal carcinoma (NPC) is caused by Epstein-Barr virus (EBV) infection, yet incorporating measurement of EBV DNA levels into clinical practice remains a challenge. Here, we summarize the relationship between NPC and EBV infection before describing the use of cell-free DNA as a plasma biomarker in cancer. We then compare conventional polymerase chain reaction methods and emerging next-generation sequencing approaches for EBV viral load detection emphasizing their prognostic and predictive utility. We conclude by considering how assay standardization, novel molecular approaches, and alternative clinical specimens may be leveraged to bring EBV testing into the routine care of patients with NPC.
鼻咽癌（NPC）是由 Epstein-Barr 病毒（EBV）感染引起的，但将 EBV DNA 水平的测量纳入临床实践仍然具有挑战性。在这里，我们总结了 NPC 与 EBV 感染之间的关系，然后描述了游离 DNA 作为癌症的血浆生物标志物的应用。接着，我们比较了传统聚合酶链反应方法和新兴的下一代测序方法在 EBV 病毒载量检测方面的应用，强调了它们在预后和预测方面的效用。最后，我们考虑了如何利用检测标准化、新的分子方法和替代临床标本，将 EBV 检测纳入 NPC 患者的常规护理中。
