Witting C
Beitr Pathol. 1977 Nov;161(3):288-91. doi: 10.1016/s0005-8165(77)80084-x.
In an attempt to apply immunomorphologic techniques to formalin-fixed material, various tissues were studied by indirect immunofluorescence. To minimize nonspecific background staining, formalin-fixed paraffin sections were treated with pronase prior to incubation with the antisera. This technique was published by Huang in 1975 to study hepatitis B core and surface antigen in formalin-fixed liver-tissue. Our examination demonstrate that this technique might enable the morphologist to identify immunoglobulins in formalin-fixed tissues and so to get new criteria for their diagnosis.
为了将免疫形态学技术应用于福尔马林固定的材料，通过间接免疫荧光对各种组织进行了研究。为了尽量减少非特异性背景染色，在与抗血清孵育之前，先用链霉蛋白酶处理福尔马林固定的石蜡切片。1975年黄发表了这项技术，用于研究福尔马林固定肝组织中的乙肝核心抗原和表面抗原。我们的检查表明，这项技术可能使形态学家能够识别福尔马林固定组织中的免疫球蛋白，从而获得新的诊断标准。
