石蜡包埋肝组织中乙肝核心抗原（HBcAg）显示的改良方法。
An improved method for HBcAg demonstration in paraffin-embedded liver tissue.
作者信息
Trevisan A, Gudat F, Busachi C, Stöcklin E, Bianchi L
出版信息
Liver. 1982 Dec;2(4):331-9. doi: 10.1111/j.1600-0676.1982.tb00831.x.
Factors influencing the preservation of HBcAg in formalin-fixed, paraffin-embedded liver tissue have been analysed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti-HBc proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24-48 h, the use of wax with a low melting point, a heating step prior to deparaffinization, and a search for anti-HBc specifically reactive for paraffinated HBcAg are recommended.
已对影响福尔马林固定、石蜡包埋肝组织中乙肝核心抗原（HBcAg）保存的因素进行了分析。固定时间、包埋介质的选择、脱石蜡处理以及抗HBc的选择被证明是关键变量。为获得最佳结果，建议使用4%缓冲福尔马林固定不超过24 - 48小时，使用低熔点蜡，在脱石蜡处理前进行加热步骤，并寻找对石蜡包埋的HBcAg具有特异性反应的抗HBc。
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