Zhang Weiwei, Liu Wen, He Shanwen, Chen Qingchu, Han Jigang, Zhang Qingfei
Key Laboratory of National Forestry and Grassland Administration on Ecological Landscaping of Challenging Urban Sites Shanghai Academy of Landscape Architecture Science and Planning Shanghai China.
Shanghai Engineering Research Center of Landscaping on Challenging Urban Sites Shanghai China.
Ecol Evol. 2021 May 27;11(12):7239-7249. doi: 10.1002/ece3.7532. eCollection 2021 Jun.
Soil degradation has been found in urban forests in Shanghai, especially in the pure plantations. Mixed plantations are considered to improve soil quality because they can stimulate organic matter cycling and increase soil carbon and nutrient content. Although soil microbes play crucial roles in regulating soil biogeochemical processes, little is known about how mixed plantations affect soil microbial communities, including bacteria, archaea, and fungi. Here, we evaluated soil chemical properties, abundances and compositions of soil bacterial, archaeal, and fungal communities, and enzyme activities in pure and mixed and plantations, located in Shanghai, China. The results showed that soil available phosphorus content in the mixed plantation of and was significantly higher than that in pure plantations, while no significant difference was observed in the content of soil organic carbon, total and available nitrogen, total and available potassium among the three studied plantations. We found higher fungal abundance in the mixed plantation, when compared to both pure plantations. Moreover, fungal abundance was positively correlated with the content of soil available phosphorus. No significant difference was found in the abundance and diversity of bacterial and archaeal community among the three studied plantations. A similarity analysis (ANOSIM) showed that mixed plantation significantly altered the community composition of archaea and fungi, accompanied with an increase of alkaline phosphatase activity. However, ANOSIM analysis of bacterial communities showed that there was no significant group separation among different plantations. Overall, results from this study indicated that fungal and archaeal communities were more sensitive to aboveground tree species than bacterial community. Moreover, mixed plantations significantly increased the activity of alkaline phosphatase and the content of soil available phosphorus, suggesting that afforestation with and is an effective way to alleviate phosphorus deficiency in urban forests in Shanghai, China.
在上海的城市森林中发现了土壤退化现象，尤其是在纯人工林中。混交林被认为可以改善土壤质量，因为它们能够促进有机质循环，增加土壤碳和养分含量。尽管土壤微生物在调节土壤生物地球化学过程中发挥着关键作用，但对于混交林如何影响包括细菌、古菌和真菌在内的土壤微生物群落却知之甚少。在此，我们评估了位于中国上海的纯林和混交林中土壤的化学性质、土壤细菌、古菌和真菌群落的丰度与组成以及酶活性。结果表明，[具体树种1]和[具体树种2]混交林的土壤有效磷含量显著高于纯林，而在所研究的三种林分中，土壤有机碳、全氮和有效氮、全钾和有效钾含量均未观察到显著差异。与两个纯林相比，我们发现混交林中真菌丰度更高。此外，真菌丰度与土壤有效磷含量呈正相关。在所研究的三种林分中，细菌和古菌群落的丰度和多样性未发现显著差异。相似性分析（ANOSIM）表明，混交林显著改变了古菌和真菌的群落组成，同时碱性磷酸酶活性增加。然而，细菌群落的ANOSIM分析表明，不同林分之间没有显著的分组差异。总体而言，本研究结果表明，真菌和古菌群落比细菌群落对地上树种更为敏感。此外，混交林显著提高了碱性磷酸酶活性和土壤有效磷含量，这表明在中国上海，用[具体树种1]和[具体树种2]进行造林是缓解城市森林磷缺乏的有效途径。
