North Minzu University/Collaborative Innovation Center for Food Production and Safety, Yinchuan, China.
Ningxia University/School of Agriculture, Yinchuan, China.
J Anim Physiol Anim Nutr (Berl). 2022 Jul;106(4):733-741. doi: 10.1111/jpn.13599. Epub 2021 Jun 29.
The purpose of this study was to investigate the effect of the skeletal muscle satellite cells (SMSCs) on the lipid deposition of the intramuscular preadipocytes (IMPs) in a co-culture system of the Tan sheep cells. The SMSCs and IMPs from Tan sheep were separated and cultured. After the two kinds of cells were separated and cultured, they were inoculated onto a transwell cell chamber co-culture plate for co-cultivation. When the cell density reached more than 90%, the cells were induced to differentiate. After the induction of the SMSCs differentiation for 8 days, the level of the IMPs differentiation and the expression levels of the differentiation marker genes and the key enzymes of the lipid metabolism were assessed. The results showed that the number and area of the lipid droplets in the IMPs in the co-culture system were significantly reduced compared to those in the IMPs culture alone (p < 0.05). Meanwhile, the expression levels of the PPARγ, c/EBPα, ACC, FAS mRNA in the IMPs were significantly decreased (p < 0.05); the expression level of aP2 mRNA was decreased, but the difference was not significant (p > 0.05).These findings indicate that the SMSCs of the Tan sheep in the co-culture system inhibited the lipid deposition by the IMPs.
本研究旨在探讨在滩羊细胞共培养体系中，骨骼肌卫星细胞（SMSCs）对肌内前体脂肪细胞（IMPs）脂质沉积的影响。分离培养滩羊的 SMSCs 和 IMPs。两种细胞分离培养后，接种于 Transwell 细胞室共培养板进行共培养。当细胞密度超过 90%时，进行诱导分化。诱导 SMSCs 分化 8 天后，评估 IMPs 分化水平以及分化标记基因和脂质代谢关键酶的表达水平。结果表明，与 IMPs 单独培养相比，共培养体系中 IMPs 中的脂质滴数量和面积明显减少（p<0.05）。同时，IMP 中 PPARγ、c/EBPα、ACC、FAS mRNA 的表达水平显著降低（p<0.05）；aP2 mRNA 的表达水平降低，但差异不显著（p>0.05）。这些发现表明，滩羊 SMSCs 在共培养体系中抑制了 IMPs 的脂质沉积。
