Yan Wei, Kan Xiangdong, Wang Yutao, Zhang Yonghao
School of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China.
Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Science, Lhasa, China.
J Anim Physiol Anim Nutr (Berl). 2023 Mar;107(2):444-452. doi: 10.1111/jpn.13750. Epub 2022 Jun 26.
The intramuscular fat (IMF) content in sheep is associated with IMF deposition, which is affected by intramuscular adipocyte hypertrophy. In this study, we established an in vitro high glucose model of intramuscular adipocytes of sheep to investigate the expression of cannabinoid receptor 1 (CB1) gene, fatty acid-binding protein 4 (FABP4) gene, lipid metabolism-associated genes (acetyl-CoA carboxylase [ACC], fatty acid synthase [FAS], and stearoyl-CoA desaturase 1 [SCD1]), and transcription factors (liver X receptor [LXRα]), sterol regulatory element-binding transcription factor 1 [SREBF-1], and carbohydrate-responsive element-binding protein [ChREBP]) as well as the changes in the lipid and triglyceride (TG) levels in intramuscular adipocytes. The results showed that the differentiated mature adipocytes had a spherical shape, and the number and volume of the lipid droplets gradually increased over time under high glucose conditions. The lipid and TG levels in intramuscular adipocytes of sheep continuously increased under high glucose conditions. Furthermore, CB1, FABP4, ACC, FAS, SCD1, LXRα, SREBF-1, and ChREBP were highly expressed under high glucose conditions, suggesting that the energetic nutrients also affect the expression of the CB1 gene, which works in coordination with lipid metabolism-associated genes and are beneficial for lipid deposition in the intramuscular adipocytes of sheep.
绵羊的肌内脂肪（IMF）含量与IMF沉积有关，而IMF沉积受肌内脂肪细胞肥大的影响。在本研究中，我们建立了绵羊肌内脂肪细胞的体外高糖模型，以研究大麻素受体1（CB1）基因、脂肪酸结合蛋白4（FABP4）基因、脂质代谢相关基因（乙酰辅酶A羧化酶[ACC]、脂肪酸合酶[FAS]和硬脂酰辅酶A去饱和酶1[SCD1]）以及转录因子（肝脏X受体[LXRα]、固醇调节元件结合转录因子1[SREBF-1]和碳水化合物反应元件结合蛋白[ChREBP]）的表达，以及肌内脂肪细胞中脂质和甘油三酯（TG）水平的变化。结果表明，分化成熟的脂肪细胞呈球形，在高糖条件下，脂滴数量和体积随时间逐渐增加。高糖条件下绵羊肌内脂肪细胞中的脂质和TG水平持续升高。此外，CB1、FABP4、ACC、FAS、SCD1、LXRα、SREBF-1和ChREBP在高糖条件下高表达，表明能量营养物质也影响CB1基因的表达，其与脂质代谢相关基因协同作用，有利于绵羊肌内脂肪细胞中的脂质沉积。
