Kimura K, LaRosa C A, Money S R, Jaffe B M
Department of Surgery, State University of New York Health Science Center, Brooklyn 11203.
J Surg Res. 1988 Oct;45(4):349-56. doi: 10.1016/0022-4804(88)90130-8.
Segmental intestinal transplantation was studied in a rat model of severe short gut syndrome across major histoincompatibility barriers. Lewis (RT1l) recipient rats whose entire small bowel (approximately 80 cm), ileocecal valve, and cecum were resected and who had no transplant, uniformly died of malabsorption on Day 9.8 +/- 0.4 (n = 11). Without cyclosporine, allograft recipients (n = 2), died of rejection on Days 8 and 10. Recipient animals with 20-cm jejunum and 40-cm jejunal transplants from Buffalo (RT1b) rats and treated daily with cyclosporine (5 mg/kg/day) intramuscularly (Days 0-28) and vitamin B12 (every other week) enjoyed significantly prolonged survival to Day 58.2 +/- 13.7, P less than 0.003, n = 10, and Day 129.1 +/- 7.4, P less than 0.001, n = 10, respectively. While 7 of 10 rats in the 20-cm jejunal transplant group died of malabsorption between Days 14 and 58, none of 10 animals in the 40-cm jejunal transplant group died of this complication. Four of 10 rats in the 40-cm jejunal transplant group thrived at 150 days after the operation, at which time they were sacrificed. Morphologically, the grafts demonstrated hypertrophic changes. The data from this study suggest that intestinal allografts have pronounced intestinal adaptative characteristics. Using segmental jejunal grafts, intestinal transplantation is an effective surgical modality for the short gut syndrome in the rat.
在跨越主要组织相容性屏障的严重短肠综合征大鼠模型中研究了节段性肠移植。Lewis（RT1l）受体大鼠的整个小肠（约80厘米）、回盲瓣和盲肠被切除且未进行移植，均在第9.8±0.4天死于吸收不良（n = 11）。在未使用环孢素的情况下，同种异体移植受体（n = 2）在第8天和第10天死于排斥反应。接受来自布法罗（RT1b）大鼠的20厘米空肠和40厘米空肠移植的受体动物，每天肌肉注射环孢素（5毫克/千克/天，第0 - 28天）和维生素B12（每隔一周），其存活时间显著延长，分别至第58.2±13.7天（P < 0.003，n = 10）和第129.1±7.4天（P < 0.001，n = 10）。虽然20厘米空肠移植组的10只大鼠中有7只在第14天至58天之间死于吸收不良，但40厘米空肠移植组的10只动物中没有一只死于这种并发症。40厘米空肠移植组的10只大鼠中有4只在术后150天茁壮成长，此时将它们处死。形态学上，移植物表现出肥大性改变。本研究数据表明，肠同种异体移植具有明显的肠道适应性特征。使用节段性空肠移植物，肠移植是大鼠短肠综合征的一种有效手术方式。
