Renal Division of Northern Jiangsu People's Hospital, Clinical Medicine College of Yangzhou University, Yangzhou, China.
Renal Division of Xi'an People's Hospital, Xi'an, China.
Front Immunol. 2021 Jun 14;12:646650. doi: 10.3389/fimmu.2021.646650. eCollection 2021.
To analyze the expression of macrophages, AIM, TGF-β1 in the kidney of IgAN patients, and to explore the role of macrophages, AIM, TGF-β1 in the progression of renal fibrosis in IgAN patients.
The paraffin specimens of renal tissue from 40 IgAN patients were selected as the observation group. At the same time, paraffin specimens of normal renal tissue from 11 patients treated by nephrectomy were selected as the normal control group. We observed the distribution of macrophages, the expression of AIM and TGF-β1 by immunohistochemical staining and/or immunofluorescence.
The number of M0, M1, M2 macrophages could be found increased in IgAN patients. M0 macrophages are mainly polarized towards M2 macrophages. The expression of AIM and TGF-β1 were significantly higher in IgAN patients than in NC. M2 macrophage, AIM and TGF-β1 were positively correlated with serum creatinine and 24-hour proteinuria, but negatively correlated with eGFR. M2 macrophages, AIM, TGF-β1 were positively correlated with fibrotic area.
M2 macrophages, AIM and TGF-β1 play important roles in the process of IgAN fibrosis, and the three influence each other.
分析 IgAN 患者肾脏中巨噬细胞、AIM、TGF-β1 的表达，探讨巨噬细胞、AIM、TGF-β1 在 IgAN 患者肾纤维化进展中的作用。
选取 40 例 IgAN 患者的肾组织石蜡标本作为观察组，同时选取 11 例行肾切除术的正常肾组织石蜡标本作为正常对照组。采用免疫组化染色和/或免疫荧光法观察巨噬细胞的分布、AIM 和 TGF-β1 的表达。
IgAN 患者中可发现 M0、M1、M2 巨噬细胞数量增加。M0 巨噬细胞主要向 M2 巨噬细胞极化。IgAN 患者的 AIM 和 TGF-β1 表达明显高于 NC。M2 巨噬细胞、AIM 和 TGF-β1 与血清肌酐和 24 小时蛋白尿呈正相关，与 eGFR 呈负相关。M2 巨噬细胞、AIM、TGF-β1 与纤维化面积呈正相关。
M2 巨噬细胞、AIM 和 TGF-β1 在 IgAN 纤维化过程中发挥重要作用，三者相互影响。
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