School of Chemistry, Monash University, Clayton, VIC 3800, Australia.
Soft Matter. 2021 Jul 21;17(28):6765-6772. doi: 10.1039/d1sm00791b.
Recently, carbon dots (CDs) have attracted wide attention for their potential use as fluorescence probes in biological and analytical chemistry due to their great stability and high fluorescence quantum yields. In our work, norepinephrine (NE)-derived CDs with green luminescence and an average size of 10 nm were fabricated using a one-step hydrothermal route. As-prepared CDs show a strong emission at a wavelength of 520 nm when excited at 420 nm, and demonstrate pH and concentration dependent fluorescence behaviour. Multiple functional groups on the CDs allow their protonation/deprotonation and thus alter fluorescence intensity and peak position in different pH conditions. Prepared CDs show significant potential to be used as a live-cell imaging agent with long-term photostability. Furthermore, a simple but effective method to determine the concentration of hemoglobin (Hb) in diluted human blood samples was also developed based on the inner filter effect (IFE). The method demonstrates good linearity from 0.01-10 μM, with a limit of determination (LOD) of 52 nM.
最近，由于其出色的稳定性和高荧光量子产率，碳点（CDs）在生物和分析化学领域作为荧光探针引起了广泛关注。在我们的工作中，使用一步水热法制备了具有绿色发光和平均尺寸为 10nm 的去甲肾上腺素（NE）衍生的 CDs。所制备的 CDs 在 420nm 激发时在 520nm 处表现出强发射，并且表现出 pH 值和浓度依赖性荧光行为。CDs 上的多个官能团允许其质子化/去质子化，从而在不同 pH 条件下改变荧光强度和峰位置。所制备的 CDs 显示出作为具有长期光稳定性的活细胞成像剂的巨大潜力。此外，还基于内滤效应（IFE）开发了一种简单但有效的方法来测定稀释人血样中血红蛋白（Hb）的浓度。该方法在 0.01-10μM 范围内表现出良好的线性，检测限（LOD）为 52nM。
