Hong Woo Tae, Yang Hyun Kyoung
Industry-University Cooperation Foundation, Pukyong National University, Busan, 48513, Republic of Korea.
Major of Smart Electrical and Electronics Engineering, Pukyong National University, Busan, 48547, Republic of Korea.
J Fluoresc. 2025 Apr 25. doi: 10.1007/s10895-025-04293-z.
The demand of fluorometric detection on Fe ions and hemoglobin are highly increased in environmental detection, anemia diagnosis, and criminal investigation. To enhance the performance of fluorometric detection of mentioned analytes, good water solubility, ease of synthesis, low cost, and high quantum yields are highly required. In this paper, the morphological, structural, luminescent characteristics of CDs, and their fluorometric detection of Fe and hemoglobin were analyzed. The carbon dots (CDs) based on o-toluidine were synthesized via hydrothermal method. The CDs exhibit a two-dimensional crystallite structure with good hydrophilic properties. Also, the CDs possess blue dominant luminescence under 240 nm light excitation. The blue luminescence was reduced by increasing Fe and hemoglobin detection. Compared with other heavy metal ions, the CDs-Fe system showed high luminescence reduction. The limit of detection of Fe and hemoglobin were calculated to be 0.133 μM and 72 nM, respectively. Comparison of luminescence decay curve, overlap of analyte absorption and luminescence spectra show that the reduction of luminescence intensity is due to FRET mechanism of CDs-Fe ions and -hemoglobin. These results indicate that the CDs can be applied as a luminescent material in fluorometric detection for environmental detection, anemia diagnosis, and criminal investigation.
在环境检测、贫血诊断和刑事调查中，对铁离子和血红蛋白的荧光检测需求大幅增加。为了提高对上述分析物的荧光检测性能，人们对材料的良好水溶性、易于合成、低成本和高量子产率有很高要求。本文分析了碳点（CDs）的形态、结构、发光特性及其对铁和血红蛋白的荧光检测。基于邻甲苯胺的碳点通过水热法合成。这些碳点呈现出具有良好亲水性的二维微晶结构。此外，碳点在240nm光激发下具有以蓝色为主的发光。随着铁和血红蛋白检测量的增加，蓝色发光减弱。与其他重金属离子相比，碳点-铁体系显示出较高的发光减弱程度。计算得出铁和血红蛋白的检测限分别为0.133μM和72 nM。发光衰减曲线的比较、分析物吸收光谱和发光光谱的重叠表明，发光强度的降低是由于碳点-铁离子和-血红蛋白的荧光共振能量转移（FRET）机制。这些结果表明，碳点可作为一种发光材料应用于环境检测、贫血诊断和刑事调查的荧光检测中。
