Fernandez-Gonzalez Lorena, Kozhevnikova Valeria, Brusentsev Eugeny, Jänsch Stefanie, Amstislavsky Sergei, Jewgenow Katarina
Department of Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrent'eva 10, 630090 Novosibirsk, Russia.
Animals (Basel). 2021 Jun 27;11(7):1909. doi: 10.3390/ani11071909.
Embryo production is a routine procedure in several species. However, in felids, the effectiveness of this approach is far behind that in the majority of laboratory species. The development of a suitable environment starts with the proper composition of culture media. Therefore, for the improvement of assisted reproduction techniques and their outcome in cats, this is an urgent task. As the addition of insulin-like growth factors (IGF-I, IGF-II) or granulocyte-macrophage colony-stimulating factor (GM-CSF) was beneficial in other mammalian species, this study aims to check whether these components, combined with other factors (such as type of fertilisation or type of culture) can provide a benefit in the felid culture system in current use. Thus, these supplements, in different concentrations and combinations, were merged with the use of two fertilisation techniques and randomly assigned to single or group culturing. The results showed that the addition of IGF-I and/or GM-CSF produced an increase in morula and blastocyst rate in a single culture system. In particular, the supplementation with 20 ng/mL of IGF-I incremented the maturation rate by 10% and significantly increased the morula and blastocyst rates in single culturing. This result is especially remarkable for wild felids, where only a few oocytes and/or embryos are available.
胚胎生产在多个物种中是一项常规操作。然而，在猫科动物中，这种方法的有效性远远落后于大多数实验物种。合适环境的营造始于培养基的恰当成分。因此，对于改进猫的辅助生殖技术及其效果而言，这是一项紧迫任务。由于添加胰岛素样生长因子（IGF-I、IGF-II）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）在其他哺乳动物物种中有益，本研究旨在检验这些成分与其他因素（如受精类型或培养类型）相结合是否能在当前使用的猫科动物培养系统中带来益处。因此，将这些不同浓度和组合的补充剂与两种受精技术结合使用，并随机分配用于单培养或组培养。结果表明，在单培养系统中添加IGF-I和/或GM-CSF可提高桑椹胚和囊胚率。特别是，添加20 ng/mL的IGF-I可使成熟率提高10%，并显著提高单培养中的桑椹胚和囊胚率。这一结果对于野生猫科动物尤为显著，因为它们可用的卵母细胞和/或胚胎数量很少。
