Laboratory for Immuno Bioengineering Research and Applications, Division of Engineering, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab Emirates.
Department of Mechanical and Biomedical Engineering, New York University, 6 MetroTech Center, Brooklyn, NY 11201, USA.
Int J Mol Sci. 2021 Jun 13;22(12):6331. doi: 10.3390/ijms22126331.
As the number of manned space flights increase, studies on the effects of microgravity on the human body are becoming more important. Due to the high expense and complexity of sending samples into space, simulated microgravity platforms have become a popular way to study these effects on earth. In addition, simulated microgravity has recently drawn the attention of regenerative medicine by increasing cell differentiation capability. These platforms come with many advantages as well as limitations. A main limitation for usage of these platforms is the lack of high-throughput capability due to the use of large cell culture vessels. Therefore, there is a requirement for microvessels for microgravity platforms that limit waste and increase throughput. In this work, a microvessel for commercial cell culture plates was designed. Four 3D printable (polycarbonate (PC), polylactic acid (PLA) and resin) and castable (polydimethylsiloxane (PDMS)) materials were assessed for biocompatibility with adherent and suspension cell types. PDMS was found to be the most suitable material for microvessel fabrication, long-term cell viability and proliferation. It also allows for efficient gas exchange, has no effect on cell culture media pH and does not induce hypoxic conditions. Overall, the designed microvessel can be used on simulated microgravity platforms as a method for long-term high-throughput biomedical studies.
随着载人航天飞行次数的增加，研究微重力对人体的影响变得越来越重要。由于将样本送入太空的费用高且过程复杂，模拟微重力平台已成为在地球上研究这些影响的一种流行方法。此外，模拟微重力最近通过提高细胞分化能力引起了再生医学的关注。这些平台有许多优点和局限性。由于使用大型细胞培养容器，这些平台的一个主要限制是缺乏高通量能力。因此，需要一种用于微重力平台的微管，以限制浪费并提高通量。在这项工作中，设计了一种用于商业细胞培养板的微管。评估了四种可 3D 打印（聚碳酸酯（PC）、聚乳酸（PLA）和树脂）和可浇铸（聚二甲基硅氧烷（PDMS））材料对贴壁和悬浮细胞类型的生物相容性。结果发现 PDMS 是制造微管的最适合材料，可长期保持细胞活力和增殖。它还允许高效的气体交换，对细胞培养基 pH 值没有影响，也不会引起缺氧条件。总的来说，设计的微管可用于模拟微重力平台，作为长期高通量生物医学研究的一种方法。
